Source:http://linkedlifedata.com/resource/pubmed/id/20813134
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| Predicate | Object |
|---|---|
| rdf:type | |
| lifeskim:mentions | |
| pubmed:issue |
1-2
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| pubmed:dateCreated |
2010-11-15
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| pubmed:abstractText |
Peste des petits ruminants virus (PPRV) is the causative agent of peste des petits ruminants (PPR), an economically important viral disease of small ruminants. In this report, a one-step, single-tube, reverse transcription loop-mediated isothermal amplification (RT-LAMP) assay was developed for the detection of PPRV. A set of six LAMP primers were designed based on the matrix gene sequence of PPRV to amplify the target RNA by incubation at 63°C for 60min with Bst DNA polymerase and reverse transcriptase. The amplified products could be observed by the naked eye. The specificity of the RT-LAMP assay was validated by amplifying eight strains of PPRV isolated in different geographical areas. No cross-reactivity with other related viruses, including rinderpest virus, canine distemper virus and measles virus, was detected. The sensitivity of the assay was similar to that of real-time reverse transcription polymerase chain reaction (RT-PCR) and 10-fold higher than that of conventional RT-PCR. Twenty clinical samples were evaluated by the RT-LAMP assay, and the results were consistent with those of real-time RT-PCR. As a simple, rapid and accurate detection method, this RT-LAMP assay has important potential applications in the clinical diagnosis of PPR and the surveillance of PPRV.
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| pubmed:language |
eng
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| pubmed:journal | |
| pubmed:citationSubset |
IM
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| pubmed:chemical | |
| pubmed:status |
MEDLINE
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| pubmed:month |
Dec
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| pubmed:issn |
1879-0984
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| pubmed:author | |
| pubmed:copyrightInfo |
Copyright © 2010 Elsevier B.V. All rights reserved.
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| pubmed:issnType |
Electronic
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| pubmed:volume |
170
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| pubmed:owner |
NLM
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| pubmed:authorsComplete |
Y
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| pubmed:pagination |
37-41
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| pubmed:meshHeading |
pubmed-meshheading:20813134-Animals,
pubmed-meshheading:20813134-Base Sequence,
pubmed-meshheading:20813134-Cross Reactions,
pubmed-meshheading:20813134-Electrophoresis, Agar Gel,
pubmed-meshheading:20813134-Fluorescent Dyes,
pubmed-meshheading:20813134-Goat Diseases,
pubmed-meshheading:20813134-Goats,
pubmed-meshheading:20813134-Nucleic Acid Amplification Techniques,
pubmed-meshheading:20813134-Peste-des-Petits-Ruminants,
pubmed-meshheading:20813134-Peste-des-petits-ruminants virus,
pubmed-meshheading:20813134-RNA, Viral,
pubmed-meshheading:20813134-Reverse Transcriptase Polymerase Chain Reaction,
pubmed-meshheading:20813134-Reverse Transcription,
pubmed-meshheading:20813134-Sensitivity and Specificity,
pubmed-meshheading:20813134-Sheep,
pubmed-meshheading:20813134-Sheep Diseases,
pubmed-meshheading:20813134-Staining and Labeling
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| pubmed:year |
2010
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| pubmed:articleTitle |
Rapid detection of peste des petits ruminants virus by a reverse transcription loop-mediated isothermal amplification assay.
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| pubmed:affiliation |
China Animal Health and Epidemiology Center, Qingdao, China.
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| pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
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