Linked Life Data

20811666

Source:http://linkedlifedata.com/resource/pubmed/id/20811666

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
4
pubmed:dateCreated
2010-9-2
pubmed:abstractText
The pathogenic mechanisms responsible for inflammatory bowel disease, especially ulcerative colitis (UC), are poorly understood. In animal models, the oral administration of dextran sulfate sodium (DSS) induces colitis, which exhibits several clinical and histological features similar to UC. In addition, the longstanding administration of DSS also induces colon cancer. However, the pathogenic factors responsible for DSS-induced colitis and the subsequent colon cancer also remain unclear. In particular, there are only limited data concerning colonic epithelia cell apoptosis and proliferation in DSS-induced colitis. Therefore, we investigated the relationships between these factors. Colitis was induced in BALB/cA Jcl mice by 8 days of oral administration of standard diets containing 5% (w/w of diet) DSS. The control mice received the standard diets only. Morphological changes in the colonic mucosa were evaluated and scored by light microscopy. Apoptosis was studied by the TUNEL assay, and cell proliferation by Ki-67 immunoreactivity. The macroscopic findings showed the most severe inflammation in the distal colon. Epithelial apoptosis increased approximately 5-fold after DSS administration as compared to the controls. On the other hand, the mitotic cells decreased about half-fold as compared to the controls. Ki-67 immunohistochemistry showed that cells with cell cycle arrest at the G0 stage in the crypt increased approximately 2-fold as compared to the controls. In conclusion, the increased apoptosis and decreased proliferation might lead to a breakdown of the epithelial barrier function, and thus facilitate the mucosal invasion of intraluminal microorganisms in DSS-induced colitis.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Oct
pubmed:issn
1791-2431
pubmed:author
pubmed:issnType
Electronic
pubmed:volume
24
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
869-74
pubmed:meshHeading
pubmed:year
2010
pubmed:articleTitle
Increased apoptosis and decreased proliferation of colonic epithelium in dextran sulfate sodium-induced colitis in mice.
pubmed:affiliation
Department of Pathology, Shiga University of Medical Science, Otsu, Shiga 520-2192, Japan. yir0828@belle.shiga-med.ac.jp
pubmed:publicationType
Journal Article