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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
11
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pubmed:dateCreated |
2010-11-2
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pubmed:abstractText |
The creation of complex tissues and organs is the ultimate goal in tissue engineering. Engineered morphogenesis necessitates spatially controlled development of multiple cell types within a scaffold implant. We present a novel method to achieve this by adhering nanoparticles containing different small-interfering RNAs (siRNAs) into nanostructured scaffolds. This allows spatial retention of the RNAs within nanopores until their cellular delivery. The released siRNAs were capable of gene silencing BCL2L2 and TRIB2, in mesenchymal stem cells (MSCs), enhancing osteogenic and adipogenic differentiation, respectively. This approach for enhancing a single type of differentiation is immediately applicable to all areas of tissue engineering. Different nanoparticles localized to spatially distinct locations within a single implant allowed two different tissue types to develop in controllable areas of an implant. As a consequence of this, we predict that complex tissues and organs can be engineered by the in situ development of multiple cell types guided by spatially restricted nanoparticles.
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/Apoptosis Regulatory Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/BCL2L2 protein, human,
http://linkedlifedata.com/resource/pubmed/chemical/DNA-Activated Protein Kinase,
http://linkedlifedata.com/resource/pubmed/chemical/DNA-Binding Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/Green Fluorescent Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/Intracellular Signaling Peptides...,
http://linkedlifedata.com/resource/pubmed/chemical/Nuclear Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/Prkdc protein, mouse,
http://linkedlifedata.com/resource/pubmed/chemical/RNA, Messenger,
http://linkedlifedata.com/resource/pubmed/chemical/RNA, Small Interfering,
http://linkedlifedata.com/resource/pubmed/chemical/TRIB2 protein, human,
http://linkedlifedata.com/resource/pubmed/chemical/enhanced green fluorescent protein
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pubmed:status |
MEDLINE
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pubmed:month |
Nov
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pubmed:issn |
1525-0024
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pubmed:author |
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pubmed:issnType |
Electronic
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pubmed:volume |
18
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
2018-27
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pubmed:dateRevised |
2011-11-1
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pubmed:meshHeading |
pubmed-meshheading:20808289-Adipocytes,
pubmed-meshheading:20808289-Animals,
pubmed-meshheading:20808289-Apoptosis,
pubmed-meshheading:20808289-Apoptosis Regulatory Proteins,
pubmed-meshheading:20808289-Blotting, Western,
pubmed-meshheading:20808289-Cell Differentiation,
pubmed-meshheading:20808289-Cell Proliferation,
pubmed-meshheading:20808289-Cells, Cultured,
pubmed-meshheading:20808289-Chondrogenesis,
pubmed-meshheading:20808289-DNA-Activated Protein Kinase,
pubmed-meshheading:20808289-DNA-Binding Proteins,
pubmed-meshheading:20808289-Female,
pubmed-meshheading:20808289-Flow Cytometry,
pubmed-meshheading:20808289-Gene Silencing,
pubmed-meshheading:20808289-Green Fluorescent Proteins,
pubmed-meshheading:20808289-Humans,
pubmed-meshheading:20808289-Immunoenzyme Techniques,
pubmed-meshheading:20808289-Intracellular Signaling Peptides and Proteins,
pubmed-meshheading:20808289-Mesenchymal Stem Cells,
pubmed-meshheading:20808289-Mice,
pubmed-meshheading:20808289-Mice, Inbred NOD,
pubmed-meshheading:20808289-Mice, SCID,
pubmed-meshheading:20808289-Nanoparticles,
pubmed-meshheading:20808289-Nanostructures,
pubmed-meshheading:20808289-Nuclear Proteins,
pubmed-meshheading:20808289-Osteoblasts,
pubmed-meshheading:20808289-RNA, Messenger,
pubmed-meshheading:20808289-RNA, Small Interfering,
pubmed-meshheading:20808289-Reverse Transcriptase Polymerase Chain Reaction,
pubmed-meshheading:20808289-Tissue Engineering,
pubmed-meshheading:20808289-Tissue Scaffolds
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pubmed:year |
2010
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pubmed:articleTitle |
siRNA nanoparticle functionalization of nanostructured scaffolds enables controlled multilineage differentiation of stem cells.
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pubmed:affiliation |
Interdisciplinary Nanoscience Center, Aarhus University, Aarhus C, Denmark.
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pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
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