Source:http://linkedlifedata.com/resource/pubmed/id/20805681
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| Predicate | Object |
|---|---|
| rdf:type | |
| lifeskim:mentions | |
| pubmed:issue |
4
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| pubmed:dateCreated |
2010-12-16
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| pubmed:abstractText |
The adrenergic ?? receptor (??-AR) gene is embedded (nested) within the serotonin 5-HT? receptor (5-HT?-R) gene and these two receptors can interact at the transcriptional and post-transcriptional levels. The mouse 5-HT?-R gene contains a number of exons and codes at least four mRNA splice variants (5-HT(4(a))-R, 5-HT(4(b))-R, 5-HT(4(e))-R, 5-HT(4(f))-R), whereas the ??-AR gene is intronless. Since 5-HT?-Rs and ??-ARs can form homodimers and heterodimers and they increase intracellular cAMP levels, these receptors may be important for integrating serotonergic and noradrenergic signals at the single-neuron level. Both 5-HT?-R and ??-AR have been implicated in autism spectrum disorders, depression, and Alzheimer's disease. In the fetal brain, these receptors may mediate the effects of stress on neurodevelopmental processes. We used quantitative reverse-transcription PCR (qRT-PCR) to investigate the developmental expression of 5-HT?-R and ??-AR in the mouse telencephalon at embryonic days (E) 13-18. At E13-E14, the mRNA levels of all 5-HT?-R splice variants were very low, but by E17-E18 they increased 7-fold (5- HT(4(a))-R), 5-fold (5-HT(4(b))-R), 9-fold (5-HT(4(e))-R), and 11-fold (5-HT(4(f))-R). The expression of 5-HT(4(a))-R and 5-HT(4(b))-R was rapidly upregulated between E14 and E15, at the time when the thalamocortical projections arrive in the telencephalon. This pattern was not observed in the expression of 5-HT(4(e))-R and 5-HT(4(f))-R, the mRNA levels of which showed a steady, gradual increase from E13 to E18. The ??-AR mRNA levels were relatively high throughout the studied period of development and increased only by 70% from E13-E14 to E17-E18. These findings suggest that 5-HT?-R splice variants and ??-ARs are differentially regulated in the embryonic telencephalon and that their relative amounts may carry developmentally important information.
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| pubmed:language |
eng
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| pubmed:journal | |
| pubmed:citationSubset |
IM
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| pubmed:chemical | |
| pubmed:status |
MEDLINE
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| pubmed:issn |
1421-9859
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| pubmed:author | |
| pubmed:copyrightInfo |
Copyright © 2010 S. Karger AG, Basel.
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| pubmed:issnType |
Electronic
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| pubmed:volume |
32
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| pubmed:owner |
NLM
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| pubmed:authorsComplete |
Y
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| pubmed:pagination |
278-87
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| pubmed:meshHeading |
pubmed-meshheading:20805681-Animals,
pubmed-meshheading:20805681-Embryo, Mammalian,
pubmed-meshheading:20805681-Gene Expression,
pubmed-meshheading:20805681-Gene Expression Regulation, Developmental,
pubmed-meshheading:20805681-Mice,
pubmed-meshheading:20805681-Protein Isoforms,
pubmed-meshheading:20805681-RNA, Messenger,
pubmed-meshheading:20805681-Receptors, Adrenergic, beta-2,
pubmed-meshheading:20805681-Receptors, Serotonin, 5-HT4,
pubmed-meshheading:20805681-Reverse Transcriptase Polymerase Chain Reaction,
pubmed-meshheading:20805681-Telencephalon
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| pubmed:year |
2010
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| pubmed:articleTitle |
Quantitative mRNA analysis of serotonin 5-HT? and adrenergic ?? receptors in the mouse embryonic telencephalon.
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| pubmed:affiliation |
Department of Psychology, University of California, Santa Barbara, CA 93106-9660, USA.
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| pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
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