Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
11
pubmed:dateCreated
2010-10-15
pubmed:abstractText
Therapy with nucleoside reverse transcriptase inhibitors (NRTIs) can be associated with mitochondrial toxicity. In vitro studies have been used to predict the predisposition for and characterize the mechanisms causing mitochondrial toxicity. Metacavir (PNA) is a novel synthetic nucleoside analog for oral administration with potent and specific antiviral activity against hepatitis B virus (HBV). We assessed the potential for mitochondrial toxicity of PNA in long-term cultures of HepG2 hepatoma cells by measuring mitochondrial function (through lactate secretion), levels of mitochondrial DNA (mtDNA), and the activities of respiratory-chain complexes I to IV. Exposure of HepG2 cells to PNA at concentrations up to 50 ?M for 15 days resulted in no deleterious effect on cell proliferation, levels of lactate or mtDNA, or enzyme activities of respiratory-chain complexes I to IV. In contrast, dideoxycytosine at 10 ?M and zidovudine at 50 ?M have significant effects on cell proliferation, levels of lactate and mtDNA, and enzyme activities of respiratory-chain complexes I to IV. However, PNA at a supratherapeutic concentration of 250 ?M could result in significant alterations in the levels of mtDNA and the activities of respiratory-chain complex enzymes, revealing evidence of the potential mitochondrial toxicity of PNA. In summary, these in vitro results indicate that the potential for PNA to interfere with mitochondrial functions is low.
pubmed:commentsCorrections
http://linkedlifedata.com/resource/pubmed/commentcorrection/20805401-10681309, http://linkedlifedata.com/resource/pubmed/commentcorrection/20805401-10972629, http://linkedlifedata.com/resource/pubmed/commentcorrection/20805401-11052597, http://linkedlifedata.com/resource/pubmed/commentcorrection/20805401-11850253, http://linkedlifedata.com/resource/pubmed/commentcorrection/20805401-12558912, http://linkedlifedata.com/resource/pubmed/commentcorrection/20805401-12859329, http://linkedlifedata.com/resource/pubmed/commentcorrection/20805401-14518717, http://linkedlifedata.com/resource/pubmed/commentcorrection/20805401-14640394, http://linkedlifedata.com/resource/pubmed/commentcorrection/20805401-15797366, http://linkedlifedata.com/resource/pubmed/commentcorrection/20805401-16406476, http://linkedlifedata.com/resource/pubmed/commentcorrection/20805401-17470651, http://linkedlifedata.com/resource/pubmed/commentcorrection/20805401-18056280, http://linkedlifedata.com/resource/pubmed/commentcorrection/20805401-1847791, http://linkedlifedata.com/resource/pubmed/commentcorrection/20805401-1851960, http://linkedlifedata.com/resource/pubmed/commentcorrection/20805401-1892364, http://linkedlifedata.com/resource/pubmed/commentcorrection/20805401-19915582, http://linkedlifedata.com/resource/pubmed/commentcorrection/20805401-2320079, http://linkedlifedata.com/resource/pubmed/commentcorrection/20805401-7695256, http://linkedlifedata.com/resource/pubmed/commentcorrection/20805401-7860738, http://linkedlifedata.com/resource/pubmed/commentcorrection/20805401-8154877, http://linkedlifedata.com/resource/pubmed/commentcorrection/20805401-8745244, http://linkedlifedata.com/resource/pubmed/commentcorrection/20805401-8813297, http://linkedlifedata.com/resource/pubmed/commentcorrection/20805401-9168161, http://linkedlifedata.com/resource/pubmed/commentcorrection/20805401-9267960, http://linkedlifedata.com/resource/pubmed/commentcorrection/20805401-942051, http://linkedlifedata.com/resource/pubmed/commentcorrection/20805401-9792373
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Nov
pubmed:issn
1098-6596
pubmed:author
pubmed:issnType
Electronic
pubmed:volume
54
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
4887-92
pubmed:dateRevised
2011-7-28
pubmed:meshHeading
pubmed:year
2010
pubmed:articleTitle
In vitro mitochondrial toxicity of metacavir, a new nucleoside reverse transcriptase inhibitor for treatment of hepatitis B virus.
pubmed:affiliation
Jiangsu Center for New Drug Screening and National Drug Screening Laboratory, China Pharmaceutical University, Nanjing, People's Republic of China.
pubmed:publicationType
Journal Article, Research Support, Non-U.S. Gov't