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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
11
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pubmed:dateCreated |
2010-10-11
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pubmed:abstractText |
Acquired resistance to tamoxifen (TAM) is a serious therapeutic problem among breast cancer patients. Previously, we have reported that TAM-resistant MCF-7 cells (TAMR-MCF-7 cells) showed increased angiogenic intensity through Pin1-dependent vascular endothelial growth factor (VEGF) production. Among six flavonoids tested in the current study, VEGF gene transcription in MCF-7 cells with stable Pin1 overexpression was inhibited most effectively by quercetin. Reporter gene assays using minimal reporters containing hypoxia response elements and activator protein-1 (AP-1) elements revealed that the activities of hypoxia inducible factor-1? (HIF-1?) and AP-1, key transcription factors for VEGF gene transcription, were suppressed by quercetin. Western blot analyses confirmed that the increased nuclear levels of c-Jun and HIF-1? in TAMR-MCF-7 cells were blocked by quercetin. Moreover, quercetin inhibited the enhanced VEGF secretion and Pin1 expression in TAMR-MCF-7 cells, which was dependent on its phosphatidyl inositol 3-kinase inhibiting effect. Chick chorioallantoic membrane assays demonstrated that the enhanced angiogenesis intensity of TAMR-MCF-7 cells was also suppressed significantly by quercetin. These results demonstrate that quercetin may have therapeutic potential for the treatment of TAM-resistant breast cancer via Pin1 inhibition.
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/Angiogenesis Inhibitors,
http://linkedlifedata.com/resource/pubmed/chemical/Antineoplastic Agents, Hormonal,
http://linkedlifedata.com/resource/pubmed/chemical/Antioxidants,
http://linkedlifedata.com/resource/pubmed/chemical/NIMA-interacting peptidylprolyl...,
http://linkedlifedata.com/resource/pubmed/chemical/Peptidylprolyl Isomerase,
http://linkedlifedata.com/resource/pubmed/chemical/Quercetin,
http://linkedlifedata.com/resource/pubmed/chemical/Tamoxifen,
http://linkedlifedata.com/resource/pubmed/chemical/VEGFA protein, human,
http://linkedlifedata.com/resource/pubmed/chemical/Vascular Endothelial Growth Factor A
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pubmed:status |
MEDLINE
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pubmed:month |
Nov
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pubmed:issn |
1873-6351
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pubmed:author |
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pubmed:copyrightInfo |
Copyright © 2010 Elsevier Ltd. All rights reserved.
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pubmed:issnType |
Electronic
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pubmed:volume |
48
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
3227-34
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pubmed:meshHeading |
pubmed-meshheading:20804812-Angiogenesis Inhibitors,
pubmed-meshheading:20804812-Animals,
pubmed-meshheading:20804812-Antineoplastic Agents, Hormonal,
pubmed-meshheading:20804812-Antioxidants,
pubmed-meshheading:20804812-Blood Vessels,
pubmed-meshheading:20804812-Breast Neoplasms,
pubmed-meshheading:20804812-Cell Line, Tumor,
pubmed-meshheading:20804812-Chick Embryo,
pubmed-meshheading:20804812-Chorioallantoic Membrane,
pubmed-meshheading:20804812-Drug Resistance, Neoplasm,
pubmed-meshheading:20804812-Female,
pubmed-meshheading:20804812-Gene Expression Regulation, Neoplastic,
pubmed-meshheading:20804812-Humans,
pubmed-meshheading:20804812-Neovascularization, Pathologic,
pubmed-meshheading:20804812-Peptidylprolyl Isomerase,
pubmed-meshheading:20804812-Quercetin,
pubmed-meshheading:20804812-Tamoxifen,
pubmed-meshheading:20804812-Vascular Endothelial Growth Factor A
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pubmed:year |
2010
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pubmed:articleTitle |
Inhibition of angiogenesis by quercetin in tamoxifen-resistant breast cancer cells.
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pubmed:affiliation |
College of Pharmacy, Seoul National University, Seoul, Republic of Korea.
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pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
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