Linked Life Data

20801631

Source:http://linkedlifedata.com/resource/pubmed/id/20801631

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PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
6
pubmed:dateCreated
2011-5-16
pubmed:abstractText
The effects of a diet rich in saturated fat on fatty liver formation and the related mechanisms that induce fatty liver were examined. C57BL/6J mice were fed butter or safflower oil as a high-fat (HF) diet (40% fat calories) for 2, 4, 10, or 17 weeks. Although both HF diets induced similar levels of obesity, HF butter-fed mice showed a two to threefold increase in liver triacylglycerol (TG) concentration compared to HF safflower oil-fed mice at 4 or 10 weeks without hyperinsulinemia. At 4 weeks, increases in peroxisome proliferator-activated receptor ?2 (PPAR?2), CD36, and adipose differentiation-related protein (ADRP) mRNAs were observed in HF butter-fed mice; at 10 weeks, an increase in sterol regulatory element-binding protein-1c (SREBP-1c) was observed; at 17 weeks, these increases were attenuated. At 4 weeks, a single injection of adenoviral vector-based short hairpin interfering RNA against PPAR?2 in HF butter-fed mice reduced PPAR? protein and mRNA of its target genes (CD36 and ADRP) by 43%, 43%, and 39%, respectively, with a reduction in liver TG concentration by 38% in 5 days. PPAR?2 knockdown also reduced mRNAs in lipogenic genes (fatty-acid-synthase, stearoyl-CoA desaturase 1, acetyl-CoA carboxylase 1) without alteration of SREBP-1c mRNA. PPAR?2 knockdown reduced mRNAs in genes related to inflammation (CD68, interleukin-1?, tumor necrosis factor-?, and monocyte chemoattractant protein-1). In conclusion, saturated fatty acid-rich oil induced fatty liver in mice, and this was triggered initially by an increase in PPAR?2 protein in the liver, which led to increased expression of lipogenic genes. Inactivation of PPAR?2 may improve fatty liver induced by HF saturated fat.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Jun
pubmed:issn
1873-4847
pubmed:author
pubmed:copyrightInfo
Copyright © 2011 Elsevier Inc. All rights reserved.
pubmed:issnType
Electronic
pubmed:volume
22
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
543-53
pubmed:meshHeading
pubmed:year
2011
pubmed:articleTitle
An increase in liver PPAR?2 is an initial event to induce fatty liver in response to a diet high in butter: PPAR?2 knockdown improves fatty liver induced by high-saturated fat.
pubmed:affiliation
Nutritional Science Program, National Institute of Health and Nutrition, Tokyo 162-8636, Japan. tomo0322@nih.go.jp
pubmed:publicationType
Journal Article, Research Support, Non-U.S. Gov't