|
rdf:type |
|
|
lifeskim:mentions |
|
|
pubmed:issue |
6
|
|
pubmed:dateCreated |
2010-9-24
|
|
pubmed:abstractText |
Alternative splicing (AS) of pre-mRNA is utilized by higher eukaryotes to achieve increased transcriptome and proteomic complexity. The serine/arginine (SR) splicing factors regulate tissue- or cell-type-specific AS in a concentration- and phosphorylation-dependent manner. However, the mechanisms that modulate the cellular levels of active SR proteins remain to be elucidated. In the present study, we provide evidence for a role for the long nuclear-retained regulatory RNA (nrRNA), MALAT1 in AS regulation. MALAT1 interacts with SR proteins and influences the distribution of these and other splicing factors in nuclear speckle domains. Depletion of MALAT1 or overexpression of an SR protein changes the AS of a similar set of endogenous pre-mRNAs. Furthermore, MALAT1 regulates cellular levels of phosphorylated forms of SR proteins. Taken together, our results suggest that MALAT1 regulates AS by modulating the levels of active SR proteins. Our results further highlight the role for an nrRNA in the regulation of gene expression.
|
|
pubmed:commentsCorrections |
|
|
pubmed:language |
eng
|
|
pubmed:journal |
|
|
pubmed:citationSubset |
IM
|
|
pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/DNA-Binding Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/Nuclear Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/PRPF6 protein, human,
http://linkedlifedata.com/resource/pubmed/chemical/RNA, Untranslated,
http://linkedlifedata.com/resource/pubmed/chemical/RNA Precursors,
http://linkedlifedata.com/resource/pubmed/chemical/RNA-Binding Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/Regulatory Sequences, Ribonucleic...,
http://linkedlifedata.com/resource/pubmed/chemical/SON protein, human,
http://linkedlifedata.com/resource/pubmed/chemical/Transcription Factors,
http://linkedlifedata.com/resource/pubmed/chemical/serine-arginine-rich splicing...
|
|
pubmed:status |
MEDLINE
|
|
pubmed:month |
Sep
|
|
pubmed:issn |
1097-4164
|
|
pubmed:author |
pubmed-author:BennettC FrankCF,
pubmed-author:BlencoweBenjamin JBJ,
pubmed-author:BubulyaPaula APA,
pubmed-author:EllisJonathan DJD,
pubmed-author:FoxH PHP,
pubmed-author:FreierSusan MSM,
pubmed-author:PrasanthKannanganattu VKV,
pubmed-author:PrasanthSupriya GSG,
pubmed-author:SharmaAlokA,
pubmed-author:ShenZhenZ,
pubmed-author:SongDavid YDY,
pubmed-author:TripathiVidishaV,
pubmed-author:WattAndrew TAT
|
|
pubmed:copyrightInfo |
Copyright © 2010 Elsevier Inc. All rights reserved.
|
|
pubmed:issnType |
Electronic
|
|
pubmed:day |
24
|
|
pubmed:volume |
39
|
|
pubmed:owner |
NLM
|
|
pubmed:authorsComplete |
Y
|
|
pubmed:pagination |
925-38
|
|
pubmed:meshHeading |
pubmed-meshheading:20797886-Alternative Splicing,
pubmed-meshheading:20797886-Animals,
pubmed-meshheading:20797886-Binding Sites,
pubmed-meshheading:20797886-Cell Line,
pubmed-meshheading:20797886-Cell Nucleus,
pubmed-meshheading:20797886-DNA-Binding Proteins,
pubmed-meshheading:20797886-HeLa Cells,
pubmed-meshheading:20797886-Humans,
pubmed-meshheading:20797886-Intranuclear Space,
pubmed-meshheading:20797886-Mice,
pubmed-meshheading:20797886-Mitosis,
pubmed-meshheading:20797886-Nuclear Proteins,
pubmed-meshheading:20797886-Phosphorylation,
pubmed-meshheading:20797886-Protein Binding,
pubmed-meshheading:20797886-Protein Interaction Domains and Motifs,
pubmed-meshheading:20797886-RNA, Untranslated,
pubmed-meshheading:20797886-RNA Precursors,
pubmed-meshheading:20797886-RNA-Binding Proteins,
pubmed-meshheading:20797886-Regulatory Sequences, Ribonucleic Acid,
pubmed-meshheading:20797886-Transcription Factors
|
|
pubmed:year |
2010
|
|
pubmed:articleTitle |
The nuclear-retained noncoding RNA MALAT1 regulates alternative splicing by modulating SR splicing factor phosphorylation.
|
|
pubmed:affiliation |
Department of Cell and Developmental Biology, University of Illinois at Urbana-Champaign, Urbana, IL 61801, USA.
|
|
pubmed:publicationType |
Journal Article,
Research Support, U.S. Gov't, Non-P.H.S.,
Research Support, Non-U.S. Gov't
|
