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rdf:type |
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lifeskim:mentions |
umls-concept:C0017262,
umls-concept:C0017337,
umls-concept:C0018061,
umls-concept:C0081620,
umls-concept:C0185117,
umls-concept:C0851285,
umls-concept:C1335073,
umls-concept:C1417833,
umls-concept:C1417834,
umls-concept:C2698420,
umls-concept:C2911684
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pubmed:issue |
1-2
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pubmed:dateCreated |
2010-10-11
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pubmed:abstractText |
Normal sexual development and reproductive function depend on precise temporal and quantitative expression of the pituitary gonadotropins, LH and FSH. LH?-subunit gene expression is achieved by transcription factors acting at highly conserved and closely spaced cis-elements in the proximal 200 base pairs of the promoter. We now demonstrate that LH? promoter activity is further regulated by the orphan nuclear receptors, chicken ovalbumin upstream promoter-transcription factors (COUP-TFI and COUP-TFII). These data establish that COUP-TFs are expressed in primary pituitary gonadotropes and two gonadotrope-derived cell lines. COUP-TFs bind to two promoter regions in the LH? gene which overlap but are distinct from two previously defined cis-elements for another orphan nuclear receptor, steroidogenic factor-1 (SF-1). Transient transfection studies demonstrated that COUP-TFs stimulate LH? gene promoter activity in the absence of SF-1, but blunt SF-1-mediated stimulation of gene expression in a reporter construct containing both SF-1 cis-elements (GSEs). Evaluation of constructs containing mutations or truncations in the GSEs revealed a complex pattern of activation and inhibition by COUP-TF on this promoter, suggesting multiple mechanisms by which this factor modulates LH? gene expression. To our knowledge, these data are the first to demonstrate COUP-TF expression and function in pituitary gonadotropes.
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/COUP Transcription Factor I,
http://linkedlifedata.com/resource/pubmed/chemical/COUP Transcription Factor II,
http://linkedlifedata.com/resource/pubmed/chemical/DNA,
http://linkedlifedata.com/resource/pubmed/chemical/Luteinizing Hormone, beta Subunit,
http://linkedlifedata.com/resource/pubmed/chemical/Protein Subunits,
http://linkedlifedata.com/resource/pubmed/chemical/RNA, Small Interfering,
http://linkedlifedata.com/resource/pubmed/chemical/Receptors, LHRH,
http://linkedlifedata.com/resource/pubmed/chemical/Steroidogenic Factor 1
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pubmed:status |
MEDLINE
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pubmed:month |
Dec
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pubmed:issn |
1872-8057
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pubmed:author |
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pubmed:copyrightInfo |
Copyright © 2010 Elsevier Ireland Ltd. All rights reserved.
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pubmed:issnType |
Electronic
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pubmed:day |
15
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pubmed:volume |
330
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
59-71
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pubmed:meshHeading |
pubmed-meshheading:20797425-Animals,
pubmed-meshheading:20797425-Base Sequence,
pubmed-meshheading:20797425-Binding, Competitive,
pubmed-meshheading:20797425-COUP Transcription Factor I,
pubmed-meshheading:20797425-COUP Transcription Factor II,
pubmed-meshheading:20797425-Cell Line,
pubmed-meshheading:20797425-Chickens,
pubmed-meshheading:20797425-Chromatin Immunoprecipitation,
pubmed-meshheading:20797425-DNA,
pubmed-meshheading:20797425-Gene Expression Regulation,
pubmed-meshheading:20797425-Gonadotrophs,
pubmed-meshheading:20797425-Humans,
pubmed-meshheading:20797425-Luteinizing Hormone, beta Subunit,
pubmed-meshheading:20797425-Mice,
pubmed-meshheading:20797425-Molecular Sequence Data,
pubmed-meshheading:20797425-Mutation,
pubmed-meshheading:20797425-Promoter Regions, Genetic,
pubmed-meshheading:20797425-Protein Binding,
pubmed-meshheading:20797425-Protein Subunits,
pubmed-meshheading:20797425-Protein Transport,
pubmed-meshheading:20797425-RNA, Small Interfering,
pubmed-meshheading:20797425-Rats,
pubmed-meshheading:20797425-Receptors, LHRH,
pubmed-meshheading:20797425-Steroidogenic Factor 1
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pubmed:year |
2010
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pubmed:articleTitle |
The orphan nuclear receptors COUP-TFI and COUP-TFII regulate expression of the gonadotropin LH? gene.
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pubmed:affiliation |
Division of Reproductive Endocrinology, Department of Obstetrics and Gynecology, University of Texas Southwestern Medical Center, Dallas, TX 75390-9032, USA.
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pubmed:publicationType |
Journal Article
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