| pubmed-article:2079503 | rdf:type | pubmed:Citation | lld:pubmed |
| pubmed-article:2079503 | lifeskim:mentions | umls-concept:C1451270 | lld:lifeskim |
| pubmed-article:2079503 | lifeskim:mentions | umls-concept:C0204727 | lld:lifeskim |
| pubmed-article:2079503 | lifeskim:mentions | umls-concept:C0205409 | lld:lifeskim |
| pubmed-article:2079503 | lifeskim:mentions | umls-concept:C0023209 | lld:lifeskim |
| pubmed-article:2079503 | lifeskim:mentions | umls-concept:C0008565 | lld:lifeskim |
| pubmed-article:2079503 | lifeskim:mentions | umls-concept:C0679622 | lld:lifeskim |
| pubmed-article:2079503 | lifeskim:mentions | umls-concept:C1998793 | lld:lifeskim |
| pubmed-article:2079503 | lifeskim:mentions | umls-concept:C0205314 | lld:lifeskim |
| pubmed-article:2079503 | lifeskim:mentions | umls-concept:C0998041 | lld:lifeskim |
| pubmed-article:2079503 | pubmed:issue | 2 | lld:pubmed |
| pubmed-article:2079503 | pubmed:dateCreated | 1991-4-26 | lld:pubmed |
| pubmed-article:2079503 | pubmed:abstractText | The isolation and purification of novel hirudins from a crude extract of the leech Hirudinaria manillensis and their analytical characterization are reported. Initial purification by gel permeation chromatography on Sephadex G50 and anion-exchange chromatography on Q Sepharose fast-flow removed most contaminants and yielded a highly active extract. Two isohirudins (designated hirudin P6 and P18) were isolated and purified by successive reversed-phase high-performance liquid chromatography on silica-based stationary phases and anion-exchange chromatography on Mono Q. The final products were characterized by reversed-phase high-performance liquid chromatography, 252Cf plasma desorption time-of-flight mass spectrometry and capillary zone electrophoresis. The molecular masses determined by 252Cf plasma desorption mass spectrometry were 7416 dalton for hirudin P6 and 7199 dalton for hirudin P18. | lld:pubmed |
| pubmed-article:2079503 | pubmed:language | eng | lld:pubmed |
| pubmed-article:2079503 | pubmed:journal | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:2079503 | pubmed:citationSubset | IM | lld:pubmed |
| pubmed-article:2079503 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:2079503 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:2079503 | pubmed:status | MEDLINE | lld:pubmed |
| pubmed-article:2079503 | pubmed:month | Sep | lld:pubmed |
| pubmed-article:2079503 | pubmed:issn | 0021-9673 | lld:pubmed |
| pubmed-article:2079503 | pubmed:author | pubmed-author:MaschlerRR | lld:pubmed |
| pubmed-article:2079503 | pubmed:author | pubmed-author:KnechtRR | lld:pubmed |
| pubmed-article:2079503 | pubmed:author | pubmed-author:SteinerVV | lld:pubmed |
| pubmed-article:2079503 | pubmed:author | pubmed-author:RaschdorfFF | lld:pubmed |
| pubmed-article:2079503 | pubmed:author | pubmed-author:GassmannEE | lld:pubmed |
| pubmed-article:2079503 | pubmed:author | pubmed-author:GruetterMM | lld:pubmed |
| pubmed-article:2079503 | pubmed:issnType | Print | lld:pubmed |
| pubmed-article:2079503 | pubmed:day | 14 | lld:pubmed |
| pubmed-article:2079503 | pubmed:volume | 530 | lld:pubmed |
| pubmed-article:2079503 | pubmed:owner | NLM | lld:pubmed |
| pubmed-article:2079503 | pubmed:authorsComplete | Y | lld:pubmed |
| pubmed-article:2079503 | pubmed:pagination | 273-82 | lld:pubmed |
| pubmed-article:2079503 | pubmed:dateRevised | 2006-11-15 | lld:pubmed |
| pubmed-article:2079503 | pubmed:meshHeading | pubmed-meshheading:2079503-... | lld:pubmed |
| pubmed-article:2079503 | pubmed:meshHeading | pubmed-meshheading:2079503-... | lld:pubmed |
| pubmed-article:2079503 | pubmed:meshHeading | pubmed-meshheading:2079503-... | lld:pubmed |
| pubmed-article:2079503 | pubmed:meshHeading | pubmed-meshheading:2079503-... | lld:pubmed |
| pubmed-article:2079503 | pubmed:meshHeading | pubmed-meshheading:2079503-... | lld:pubmed |
| pubmed-article:2079503 | pubmed:meshHeading | pubmed-meshheading:2079503-... | lld:pubmed |
| pubmed-article:2079503 | pubmed:meshHeading | pubmed-meshheading:2079503-... | lld:pubmed |
| pubmed-article:2079503 | pubmed:meshHeading | pubmed-meshheading:2079503-... | lld:pubmed |
| pubmed-article:2079503 | pubmed:meshHeading | pubmed-meshheading:2079503-... | lld:pubmed |
| pubmed-article:2079503 | pubmed:meshHeading | pubmed-meshheading:2079503-... | lld:pubmed |
| pubmed-article:2079503 | pubmed:meshHeading | pubmed-meshheading:2079503-... | lld:pubmed |
| pubmed-article:2079503 | pubmed:year | 1990 | lld:pubmed |
| pubmed-article:2079503 | pubmed:articleTitle | Isolation and purification of novel hirudins from the leech Hirudinaria manillensis by high-performance liquid chromatography. | lld:pubmed |
| pubmed-article:2079503 | pubmed:affiliation | Pharmaceuticals Research Laboratories, Ciba-Geigy Ltd., Basel, Switzerland. | lld:pubmed |
| pubmed-article:2079503 | pubmed:publicationType | Journal Article | lld:pubmed |
