Linked Life Data

207895

Source:http://linkedlifedata.com/resource/pubmed/id/207895

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
2
pubmed:dateCreated
1978-8-28
pubmed:abstractText
Ad2(+)ND(1), a nondefective hybrid virus containing a segment of the early region of simian virus 40 (SV40) DNA covalently inserted into the human adenovirus 2 genome, enhances the growth of human adenoviruses in simian cells and induces the SV40 U antigen. This hybrid previously has been shown to code for a 28,000 (28K) molecular weight protein not present in wild-type adenovirus 2-infected cells. By radioimmunoprecipitation using sera from hamsters bearing SV40-specific tumors, we have established that the Ad2(+)ND(1)-induced 28K protein is SV40-specific. This Ad2(+)ND(1)-induced protein is synthesized as a 30K molecular weight precursor, which is detectable only when infected cells are pulse-labeled in the presence of the protease inhibitor tosylamino phenylethyl chloromethyl ketone. Upon fractionation of labeled cell extracts, about 80% of the 28K protein is found in the plasma membrane fraction, whereas the remaining 20% is associated with the outer nuclear membrane. This protein is not detectable either in the nucleus or in the cytoplasm. Blockage of proteolytic cleavage by tosylamino phenylethyl chloromethyl ketone did not alter the topographic distribution of this SV40-specific protein, although the amount of the precursor protein in the outer nuclear membrane increased fourfold while that in the plasma membrane was proportionately decreased. This result suggests that the 28K protein is transferred from the outer nuclear membrane to the plasma membrane after posttranslational cleavage of the 30K precursor polypeptide. These data offer further support to the proposal that the 28K protein contains the determinants for SV40 U antigen and is responsible for SV40 enhancement of adenovirus growth in simian cells.
pubmed:commentsCorrections
http://linkedlifedata.com/resource/pubmed/commentcorrection/207895-1102604, http://linkedlifedata.com/resource/pubmed/commentcorrection/207895-165626, http://linkedlifedata.com/resource/pubmed/commentcorrection/207895-170741, http://linkedlifedata.com/resource/pubmed/commentcorrection/207895-178903, http://linkedlifedata.com/resource/pubmed/commentcorrection/207895-183014, http://linkedlifedata.com/resource/pubmed/commentcorrection/207895-197266, http://linkedlifedata.com/resource/pubmed/commentcorrection/207895-198574, http://linkedlifedata.com/resource/pubmed/commentcorrection/207895-4311250, http://linkedlifedata.com/resource/pubmed/commentcorrection/207895-4350710, http://linkedlifedata.com/resource/pubmed/commentcorrection/207895-4355862, http://linkedlifedata.com/resource/pubmed/commentcorrection/207895-4364898, http://linkedlifedata.com/resource/pubmed/commentcorrection/207895-5358824, http://linkedlifedata.com/resource/pubmed/commentcorrection/207895-66327
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
May
pubmed:issn
0022-538X
pubmed:author
pubmed:issnType
Print
pubmed:volume
26
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
411-9
pubmed:dateRevised
2009-11-18
pubmed:meshHeading
pubmed:year
1978
pubmed:articleTitle
Biosynthesis, immunological specificity, and intracellular distribution of the simian virus 40-specific protein induced by the nondefective hybrid Ad2+ND1.
pubmed:publicationType
Journal Article