rdf:type |
|
lifeskim:mentions |
umls-concept:C0007610,
umls-concept:C0010823,
umls-concept:C0039593,
umls-concept:C0052026,
umls-concept:C0205409,
umls-concept:C0205725,
umls-concept:C0439852,
umls-concept:C0439858,
umls-concept:C1510438,
umls-concept:C1524063,
umls-concept:C1707455
|
pubmed:issue |
5
|
pubmed:dateCreated |
1978-8-28
|
pubmed:abstractText |
Use of an antigen consisting of purified isolated nuclei from a mixture of human cytomegalovirus-infected and uninfected fibroblasts in a 2:1 ratio is a simple and reliable method for eliminating nonspecific fluorescence associated with the presence of Fc-immunoglobulin G receptors in the cytoplasm of infected cells. The specificity obtained with this antigen on 100 normal human sera was 99, 100, and 98% when compared with microneutralization, anticomplement immunofluorescence, and conventional indirect fluorescent-antibody assays, respectively. Also, 95% of the antibody titers obtained with the nuclear antigen had a perfect correlation with or were within a fourfold-dilution difference of the antibody levels obtained by anticomplement immunofluorescence and the conventional indirect fluorescent-antibody test.
|
pubmed:commentsCorrections |
|
pubmed:language |
eng
|
pubmed:journal |
|
pubmed:citationSubset |
IM
|
pubmed:chemical |
|
pubmed:status |
MEDLINE
|
pubmed:month |
May
|
pubmed:issn |
0095-1137
|
pubmed:author |
|
pubmed:issnType |
Print
|
pubmed:volume |
7
|
pubmed:owner |
NLM
|
pubmed:authorsComplete |
Y
|
pubmed:pagination |
486-9
|
pubmed:dateRevised |
2009-11-18
|
pubmed:meshHeading |
pubmed-meshheading:207719-Antibodies, Viral,
pubmed-meshheading:207719-Antigens, Viral,
pubmed-meshheading:207719-Cell Nucleus,
pubmed-meshheading:207719-Complement Fixation Tests,
pubmed-meshheading:207719-Culture Techniques,
pubmed-meshheading:207719-Cytomegalovirus Infections,
pubmed-meshheading:207719-Diagnosis, Differential,
pubmed-meshheading:207719-Female,
pubmed-meshheading:207719-Fibroblasts,
pubmed-meshheading:207719-Fluorescent Antibody Technique,
pubmed-meshheading:207719-Humans,
pubmed-meshheading:207719-Neutralization Tests,
pubmed-meshheading:207719-Pregnancy,
pubmed-meshheading:207719-Pregnancy Complications, Infectious
|
pubmed:year |
1978
|
pubmed:articleTitle |
Use of isolated nuclei in the indirect fluorescent-antibody test for human cytomegalovirus infection: comparison with microneutralization, anticomplement, and conventional indirect fluorescent-antibody assays.
|
pubmed:publicationType |
Journal Article,
Comparative Study,
Research Support, U.S. Gov't, P.H.S.,
Research Support, U.S. Gov't, Non-P.H.S.
|