Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
5
pubmed:dateCreated
1978-8-28
pubmed:abstractText
Use of an antigen consisting of purified isolated nuclei from a mixture of human cytomegalovirus-infected and uninfected fibroblasts in a 2:1 ratio is a simple and reliable method for eliminating nonspecific fluorescence associated with the presence of Fc-immunoglobulin G receptors in the cytoplasm of infected cells. The specificity obtained with this antigen on 100 normal human sera was 99, 100, and 98% when compared with microneutralization, anticomplement immunofluorescence, and conventional indirect fluorescent-antibody assays, respectively. Also, 95% of the antibody titers obtained with the nuclear antigen had a perfect correlation with or were within a fourfold-dilution difference of the antibody levels obtained by anticomplement immunofluorescence and the conventional indirect fluorescent-antibody test.
pubmed:commentsCorrections
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
May
pubmed:issn
0095-1137
pubmed:author
pubmed:issnType
Print
pubmed:volume
7
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
486-9
pubmed:dateRevised
2009-11-18
pubmed:meshHeading
pubmed:year
1978
pubmed:articleTitle
Use of isolated nuclei in the indirect fluorescent-antibody test for human cytomegalovirus infection: comparison with microneutralization, anticomplement, and conventional indirect fluorescent-antibody assays.
pubmed:publicationType
Journal Article, Comparative Study, Research Support, U.S. Gov't, P.H.S., Research Support, U.S. Gov't, Non-P.H.S.