Linked Life Data

20765

Source:http://linkedlifedata.com/resource/pubmed/id/20765

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
4
pubmed:dateCreated
1977-11-25
pubmed:abstractText
Tick-borne encephalitis (TBE) virus grown in chick embryo cells and purified by rate-zonal centrifugation was shown to have a density of 1.195 g/cm3 in sucrose density gradients. Under the conditions used, CsCl centrifugation caused disruption of the virus and less than 5% of input infectivity could be recovered from the virus peak at 1.23 g/cm3. As in other fiaviviruses, three polypeptides (V1, V2, V3) were detected by polyacrylamide gel electrophoresis in radioactively labelled purified TBE virus. After disruption of TBE virus with Nonidet P-40, Tween-80/Tri-(n-butyl)-phosphate or sodium deoxycholate, a 120--130 S nucleoprotein could be separated by rate zonal centrifugation from more slowly sedimenting haemagglutinins. Immunization of mice with these haemagglutinins showed that they induced haemagglutination inhibiting, neutralizing and complement fixing antibodies. A pronounced increase in immunogenicity could be observed by the use of Al(OH3) as an adjuvant.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Jul
pubmed:issn
0001-723X
pubmed:author
pubmed:issnType
Print
pubmed:volume
21
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
308-16
pubmed:dateRevised
2004-11-17
pubmed:meshHeading
pubmed:year
1977
pubmed:articleTitle
Characterization of tick-borne encephalitis virus and immunogenicity of its surface components in mice.
pubmed:publicationType
Journal Article