Linked Life Data

20739512

Source:http://linkedlifedata.com/resource/pubmed/id/20739512

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
5
pubmed:dateCreated
2010-10-27
pubmed:abstractText
Insulin-like growth factor I (IGF-I) is a potent myogenic factor that plays a critical role in muscle regeneration and muscle hypertrophy. The purpose of this study was to evaluate the effect of IGF-I overexpression on the recovery of muscle size and function during reloading/reambulation after a period of cast immobilization in predominantly fast twitch muscles. In addition, we investigated concomitant molecular responses in IGF-I receptor and binding proteins (BPs). Recombinant adeno-associated virus vector for IGF-I (rAAV-IGF-IA) was injected into the anterior compartment of one of the hindlimbs of young (3 wk) C57BL6 female mice. At 20 wk of age, both hindlimbs were cast immobilized in a shortened position for 2 wk to unload the tibialis anterior (TA) and extensor longus digitorum (EDL) muscles. The TA and EDL muscles were removed bilaterally after 2 wk of cast immobilization and after 1 and 3 wk of free cage reambulation. Increases in IGF-I mRNA and protein levels with IGF-I overexpression were associated with significant increases in muscle wet weight, fiber size, and tetanic force, although overexpression did not protect against cast immobilization-induced muscle atrophy. After 1 wk of reambulation, evidence of enhanced muscle regeneration was noted in IGF-I-overexpressing muscles with an increased prevalence of central nuclei, embryonic myosin, and Pax7 positive fibers. We also observed larger relative gains in muscle size (wet weight and fiber area), but not force, during the 3-wk reambulation period in hindlimb muscles overexpressing IGF-I compared with contralateral control legs. Changes in IGFBP-5 mRNA expression during cast immobilization and reambulation paralleled those of IGF-I, whereas IGFBP-3 expression changed inversely to IGFBP-5.
pubmed:grant
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Nov
pubmed:issn
1522-1555
pubmed:author
pubmed:issnType
Electronic
pubmed:volume
299
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
E730-40
pubmed:dateRevised
2011-11-1
pubmed:meshHeading
pubmed-meshheading:20739512-Animals, pubmed-meshheading:20739512-Dependovirus, pubmed-meshheading:20739512-Female, pubmed-meshheading:20739512-Gene Transfer Techniques, pubmed-meshheading:20739512-Hindlimb, pubmed-meshheading:20739512-Immobilization, pubmed-meshheading:20739512-Immunohistochemistry, pubmed-meshheading:20739512-Insulin-Like Growth Factor Binding Proteins, pubmed-meshheading:20739512-Insulin-Like Growth Factor I, pubmed-meshheading:20739512-Mice, pubmed-meshheading:20739512-Mice, Inbred C57BL, pubmed-meshheading:20739512-Muscle Fibers, Fast-Twitch, pubmed-meshheading:20739512-Muscle Proteins, pubmed-meshheading:20739512-Muscular Atrophy, pubmed-meshheading:20739512-RNA, pubmed-meshheading:20739512-Random Allocation, pubmed-meshheading:20739512-Receptor, IGF Type 1, pubmed-meshheading:20739512-Reverse Transcriptase Polymerase Chain Reaction, pubmed-meshheading:20739512-Torsion, Mechanical, pubmed-meshheading:20739512-Walking
pubmed:year
2010
pubmed:articleTitle
Impact of viral-mediated IGF-I gene transfer on skeletal muscle following cast immobilization.
pubmed:affiliation
Dept. of Physical Therapy, Univ. of Florida, Gainesville, 32610, USA.
pubmed:publicationType
Journal Article, Research Support, N.I.H., Extramural