Linked Life Data

20729753

Source:http://linkedlifedata.com/resource/pubmed/id/20729753

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
5
pubmed:dateCreated
2010-11-10
pubmed:abstractText
The objective of the present study was to evaluate the efficacy and mechanism of Crataegus oxycantha (COC) extract in preventing ischemia-reperfusion (IR) injury in an in vivo rat model of acute myocardial infarction induced by a 30-minute regional ischemia followed by 72 hours of reperfusion. The COC extract [100 mg/(kg body weight)] was administered 12 hours after the surgical procedure and then at 24-hour intervals for 3 days. Animals treated with COC extract showed a significant decrease in creatine kinase activity and infarct size. At the molecular level, COC administration resulted in a significant attenuation of PTEN (phosphatase and tensin homolog deleted on chromosome 10) and upregulation of phospho-Akt and c-Raf levels in the heart. As a consequence, cleaved caspase-9 and cleaved caspase-7 levels were significantly downregulated, indicating negative regulation of apoptosis by COC extract. In part with the hypoxia-inducible factor (HIF) signaling pathway, COC extract administration significantly upregulated the prolyl hydroxylase-2 level. In contrast, other proapoptotic proteins such as nuclear factor-?B, cytochrome c, apoptosis-inducing factor, and cleaved poly(adenosine diphosphate-ribose) polymerase levels were significantly downregulated in the COC-treated group when compared with the untreated control group. The results suggested that COC extract attenuated apoptotic incidence in the experimental myocardial ischemia-reperfusion model by regulating Akt and HIF-1 signaling pathways.
pubmed:grant
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Nov
pubmed:issn
1533-4023
pubmed:author
pubmed:issnType
Electronic
pubmed:volume
56
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
526-31
pubmed:meshHeading
pubmed-meshheading:20729753-Animals, pubmed-meshheading:20729753-Apoptosis, pubmed-meshheading:20729753-Apoptosis Regulatory Proteins, pubmed-meshheading:20729753-Crataegus, pubmed-meshheading:20729753-Creatine Kinase, pubmed-meshheading:20729753-Hypoxia-Inducible Factor 1, pubmed-meshheading:20729753-Male, pubmed-meshheading:20729753-Myocardial Infarction, pubmed-meshheading:20729753-Myocardial Reperfusion Injury, pubmed-meshheading:20729753-Myocardium, pubmed-meshheading:20729753-Phytotherapy, pubmed-meshheading:20729753-Plant Extracts, pubmed-meshheading:20729753-Procollagen-Proline Dioxygenase, pubmed-meshheading:20729753-Proto-Oncogene Proteins c-akt, pubmed-meshheading:20729753-Rats, pubmed-meshheading:20729753-Rats, Sprague-Dawley, pubmed-meshheading:20729753-Signal Transduction, pubmed-meshheading:20729753-Up-Regulation
pubmed:year
2010
pubmed:articleTitle
Crataegus oxycantha extract attenuates apoptotic incidence in myocardial ischemia-reperfusion injury by regulating Akt and HIF-1 signaling pathways.
pubmed:affiliation
Department of Internal Medicine, Davis Heart and Lung Research Institute, The Ohio State University, Columbus, OH 43210, USA.
pubmed:publicationType
Journal Article, Research Support, N.I.H., Extramural