Linked Life Data

20729073

Source:http://linkedlifedata.com/resource/pubmed/id/20729073

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
4
pubmed:dateCreated
2010-12-3
pubmed:abstractText
No one technique for multiplexing 100+ label-free measurements in a single well or flowcell has yet gained wide acceptance, probably because the added complexity introduced by the multiplexing element is seen to outweigh any possible cost advantage, or the multiplexing scheme itself is not flexible enough to accommodate the desired combinations of immobilization conditions and target/analyte molecules. Here, we demonstrate a simple yet highly versatile technology which uses microparticles, each bearing both an identifier code and an optical grating, to permit the inclusion of diverse molecules such as protein A, IgG and DNA, on chemically diverse -OH, -NH(2) and -COOH-terminated surfaces, within a single flowcell assay. Binding of avidin is used to reveal the presence of the immobilized biotinylated species, and to compare directly the binding of similar molecules on dissimilar surfaces, and vice-versa. Though we report the results of a 26-plex assay here, the technique itself has scope for increased throughput up to the level of hundreds of molecular species.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Dec
pubmed:issn
1873-4235
pubmed:author
pubmed:copyrightInfo
Copyright © 2010 Elsevier B.V. All rights reserved.
pubmed:issnType
Electronic
pubmed:day
15
pubmed:volume
26
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
1719-22
pubmed:meshHeading
pubmed:year
2010
pubmed:articleTitle
Label-free genetic and proteomic marker detection within a single flowcell assay.
pubmed:affiliation
Toshiba Research Europe Limited, Cambridge Research Laboratory, 208 Cambridge Science Park, Milton Road, Cambridge, CB4 0GZ, UK.
pubmed:publicationType
Journal Article, Evaluation Studies