Linked Life Data

20725087

Source:http://linkedlifedata.com/resource/pubmed/id/20725087

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
2
pubmed:dateCreated
2011-1-10
pubmed:abstractText
Krüppel-like factors (KLFs) as a family of zinc-finger transcription factors involve in the regulation of many physiological processes. In these studies, KLF9 was characterized for its role in adipogenesis. The expression of KLF9 was markedly upregulated during the middle stage of 3T3-L1 adipocyte differentiation, and inhibition of KLF9 by RNAi impaired adipogenesis. Using promoter deletion and mutation analysis, we identified two KLF9-binding sites within the 0.6-kb region of the PPAR?2 proximal promoter, indicating that KLF9 interacts with the PPAR?2 promoter. Furthermore, we found that KLF9 could synergistically activate PPAR?2 promoter by directly interacting with C/EBP?. In addition, overexpression of PPAR?2 rescued the impairment of adipocyte differentiation induced by KLF9 knockdown, which supports that PPAR?2 is a downstream target of KLF9. Collectively, our results indicate KLF9 as a key pro-adipogenic transcription factor through regulation of PPAR?2 expression with C/EBP? at the middle stage of adipogenesis.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Feb
pubmed:issn
1476-5403
pubmed:author
pubmed:issnType
Electronic
pubmed:volume
18
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
315-27
pubmed:meshHeading
pubmed:year
2011
pubmed:articleTitle
Krüppel-like factor KLF9 regulates PPAR? transactivation at the middle stage of adipogenesis.
pubmed:affiliation
Key Laboratory of Systems Biology, Institute of Biochemistry and Cell Biology, Shanghai Institutes for Biological Sciences, Chinese Academy of Sciences, 320 Yue-Yang Road, Shanghai, China.
pubmed:publicationType
Journal Article, Research Support, Non-U.S. Gov't