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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
8
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pubmed:dateCreated |
1991-8-19
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pubmed:abstractText |
Viral particles infecting some stocks of the protozoan parasite Leishmania braziliensis subsp. guyanensis contain a double-stranded RNA genome of ca. 5 kbp and are associated with an RNA-dependent RNA polymerase which synthesizes in vitro double-stranded and single-stranded, genome-length transcripts. The majority of viral transcripts are single-stranded and templated from one genomic strand. The putative replicase generates double-stranded RNA by synthesizing the opposite strand on a preexisting RNA template. These data are compatible with a replicative cycle proposed for the yeast viruses. Purification of the Leishmania virus on CsCl yields virus without double-strand synthesis activity, while this activity is consistently present in unpurified virus and in particles from sucrose gradients. The deficiency in double-strand synthesis in CsCl-derived virions correlates with the accessibility of the viral polymerase and genomic RNA to exogenously added enzymes, indicative of a structural modification of the viral capsid.
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pubmed:grant |
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pubmed:commentsCorrections |
http://linkedlifedata.com/resource/pubmed/commentcorrection/2072450-2353455,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2072450-2355918,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2072450-2370680,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2072450-2550303,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2072450-2762308,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2072450-3068964,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2072450-3200841,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2072450-3489942,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2072450-3520572,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2072450-3807947,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2072450-6154874
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
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pubmed:status |
MEDLINE
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pubmed:month |
Aug
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pubmed:issn |
0022-538X
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:volume |
65
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
4211-5
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pubmed:dateRevised |
2009-11-18
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pubmed:meshHeading |
pubmed-meshheading:2072450-Animals,
pubmed-meshheading:2072450-Blotting, Northern,
pubmed-meshheading:2072450-Centrifugation, Density Gradient,
pubmed-meshheading:2072450-Cesium,
pubmed-meshheading:2072450-Chlorides,
pubmed-meshheading:2072450-DNA-Directed RNA Polymerases,
pubmed-meshheading:2072450-Electrophoresis, Agar Gel,
pubmed-meshheading:2072450-Leishmania braziliensis,
pubmed-meshheading:2072450-RNA, Double-Stranded,
pubmed-meshheading:2072450-RNA, Viral,
pubmed-meshheading:2072450-RNA Viruses,
pubmed-meshheading:2072450-Sucrose,
pubmed-meshheading:2072450-Virion
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pubmed:year |
1991
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pubmed:articleTitle |
Genomic structure and RNA polymerase activity in Leishmania virus.
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pubmed:affiliation |
Department of Microbiology and Molecular Genetics, Harvard Medical School, Boston, Massachusetts.
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pubmed:publicationType |
Journal Article,
Research Support, U.S. Gov't, P.H.S.,
Research Support, U.S. Gov't, Non-P.H.S.,
Research Support, Non-U.S. Gov't
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