Linked Life Data

20723308

Source:http://linkedlifedata.com/resource/pubmed/id/20723308

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
4
pubmed:dateCreated
2010-8-20
pubmed:abstractText
Fc? receptorIIIa (Fc?RIIIa) polymorphism was considered to influence clinical response to therapeutic monoclonal antibody (MAb) against cancer, which is suggested to affect MAb binding and MAb-dependent NK cell-mediated cytotoxicity. The purpose of this study was to examine the Fc?RIIIa gene polymorphisms in healthy children and in children with hematological malignancy, and to explore its possible effect on MAb in children with hematological malignancies. 43 healthy children (H) and 20 pediatric patients with hematological malignancies (HM) were enrolled in this study. DNA was isolated from peripheral blood, and then nest-polymerase chain reaction-restriction fragment length polymorphism (nest-PCR and PCR-RFLP) was used to determine the Fc?RIIIa-158 genotypes in each groups of subject, digested fragments were subjected to electrophoresis on 15% PAGE. The results showed that there were a higher frequencies of Fc?RIIIa-158V/F in H and HM group (72.1% and 75.0% respectively), the frequencies of Fc?RIIIa-158V/V were 27.9% and 25.0% in H and HM group respectively, but there was no Fc?RIIIa-158F/F in the two groups. No significant difference in distribution of the Fc?RIIIa-158 genotype was found between HM and H groups (p > 0.05). It is concluded that Fc?RIIIa-158V/F is more frequent, while Fc?RIIIa-158V/V is less, but Fc?RIIIa-158F/F is very rare in both groups. No significant difference of Fc?RIIIa polymorphism distribution is found between healthy and hematological malignancy groups.
pubmed:language
chi
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Aug
pubmed:issn
1009-2137
pubmed:author
pubmed:issnType
Print
pubmed:volume
18
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
959-62
pubmed:meshHeading
pubmed:year
2010
pubmed:articleTitle
[Fc? receptorIIIa polymorphism in healthy children and those with hematological malignancies].
pubmed:affiliation
Department of Pediatrics, Sun Yet-Sen Memorial Hospital, Sun Yet-Sen University, Guangzhou 510120, Guangdong Province, China.
pubmed:publicationType
Journal Article, English Abstract, Research Support, Non-U.S. Gov't