Linked Life Data

20723297

Source:http://linkedlifedata.com/resource/pubmed/id/20723297

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
4
pubmed:dateCreated
2010-8-20
pubmed:abstractText
This study was purposed to investigate the relationship between activation of nuclear factor-?B (NF-?B) and multidrug resistance in K562/AO? cells and its mechanism. Human erythroleukemic cell line K562 and its adriamycin-resistant counterpart K562/AO? cells were used in the study. After inhibiting the activation of NF-?B with noncytotoxic concentration of antioxidant pyrrolidine dithiocarbamate (PDTC) in vitro, the multiple of drug resistance of K562/AO? cells was assessed by MTT assay. RT-PCR and flow cytometry method were used to detect the relative expression of mdr-1 mRNA and P-gp, respectively. The results showed that (1) multidrug resistance of K562/AO? cells to ADM was 59 times higher than that of K562 cells. When being pretreated with 0.2 ?mol/L PDTC which is noncytotoxic to cells, the IC?? of ADM in K562/AO? cells was sharply decreased with relative reverse efficiency of 93.03%, which was more higher than that of classic modifying agents Verapamil (Ver); (2) NF-?B activity of K562/AO? cells was significantly higher than that of K562 cells (p < 0.01). When being treated with PDTC, the activation of NF-?B was sharply decreased in K562/AO? cells; with 0.2 ?mol/L PDTC for 24 hours it decreased to the lowest, nearly to the K562 cell level (p > 0.05); (3) the relative expression of both mdr-1 mRNA and P-gp in K562/AO? cells was more higher; the expressions of mdr-1 mRNA and P-gp both were inhibited in K562/AO? cell group treated with PDTC for 48 hours. It is concluded that the PDTC used as an inhibitor of NF-?B activity can partially reverse the multidrug resistance of K562/AO? cells, which mechanism can be associated with the down-regulation of mdr-1 mRNA and P-gp.
pubmed:language
chi
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Aug
pubmed:issn
1009-2137
pubmed:author
pubmed:issnType
Print
pubmed:volume
18
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
903-8
pubmed:meshHeading
pubmed:year
2010
pubmed:articleTitle
[Reversal effect of nuclear factor-?B protease inhibitor PDTC on multidrug resistance of K562/AO? cells and its mechanism].
pubmed:affiliation
Department of Pediatrics, Lianyungang Municipal Frist People Hospital, Lianyungang 222002, Jiangsu Province, China.
pubmed:publicationType
Journal Article, English Abstract