Linked Life Data

20723137

Source:http://linkedlifedata.com/resource/pubmed/id/20723137

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
3
pubmed:dateCreated
2011-5-6
pubmed:abstractText
The major challenge of follicular fluid proteomic analysis is the presence of high-abundance proteins that originate from plasma. These proteins can prevent the detection of lower abundant ones, produced locally by follicle cells and that may have important roles in follicular activity. In this study, the novel technology called hexapeptide ligand library was evaluated to enrich the low-abundance proteins in follicular fluid of human (HFF), porcine (PFF) and equine (EFF) prior 2D-PAGE. Our results showed that the new strategy enabled detection of many new protein spots, increased resolution and highly improved the intensity of low-abundance proteins by 2D-PAGE.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Jun
pubmed:issn
1439-0531
pubmed:author
pubmed:copyrightInfo
© 2010 Blackwell Verlag GmbH.
pubmed:issnType
Electronic
pubmed:volume
46
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
561-3
pubmed:meshHeading
pubmed:year
2011
pubmed:articleTitle
Improvement of 2D-PAGE resolution of human, porcine and equine follicular fluid by means of hexapeptide ligand library.
pubmed:affiliation
Physiologie de la Reproduction et des Comportements, Nouzilly, France.
pubmed:publicationType
Journal Article, Research Support, Non-U.S. Gov't