Source:http://linkedlifedata.com/resource/pubmed/id/20722389
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Predicate | Object |
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rdf:type | |
lifeskim:mentions | |
pubmed:issue |
10
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pubmed:dateCreated |
2010-10-4
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pubmed:abstractText |
Several lines of evidence support an autoimmune basis for alopecia areata (AA), a common putative autoimmune hair loss disorder. However, definitive support is lacking largely because the identity of hair follicle (HF) autoantigen(s) involved in its pathogenesis remains unknown. Here, we isolated AA-reactive HF-specific antigens from normal human scalp anagen HF extracts by immunoprecipitation using serum antibodies from 10 AA patients. Samples were analyzed by LC-MALDI-TOF/TOF mass spectrometry, which indicated strong reactivity to the hair growth phase-specific structural protein trichohyalin in all AA sera. Keratin 16 (K16) was also identified as another potential AA-relevant target HF antigen. Double immunofluorescence studies using AA (and control sera) together with a monoclonal antibody to trichohyalin revealed that AA sera contained immunoreactivity that colocalized with trichohyalin in the growth phase-specific inner root sheath of HF. Furthermore, a partial colocalization of AA serum reactivity with anti-K16 antibody was observed in the outer root sheath of the HF. In summary, this study supports the involvement of an immune response to anagen-specific HFs antigens in AA and specifically suggests that an immune response to trichohyalin and K16 may have a role in the pathogenesis of the enigmatic disorder.
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pubmed:language |
eng
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pubmed:journal | |
pubmed:citationSubset |
IM
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pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/Autoantigens,
http://linkedlifedata.com/resource/pubmed/chemical/Intermediate Filament Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/Keratin-16,
http://linkedlifedata.com/resource/pubmed/chemical/Protein Precursors,
http://linkedlifedata.com/resource/pubmed/chemical/trichohyalin
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pubmed:status |
MEDLINE
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pubmed:month |
Oct
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pubmed:issn |
1535-3907
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pubmed:author | |
pubmed:issnType |
Electronic
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pubmed:day |
1
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pubmed:volume |
9
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
5153-63
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pubmed:dateRevised |
2011-11-17
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pubmed:meshHeading |
pubmed-meshheading:20722389-Adult,
pubmed-meshheading:20722389-Alopecia Areata,
pubmed-meshheading:20722389-Autoantigens,
pubmed-meshheading:20722389-Female,
pubmed-meshheading:20722389-Fluorescent Antibody Technique, Indirect,
pubmed-meshheading:20722389-Hair Follicle,
pubmed-meshheading:20722389-Humans,
pubmed-meshheading:20722389-Intermediate Filament Proteins,
pubmed-meshheading:20722389-Keratin-16,
pubmed-meshheading:20722389-Male,
pubmed-meshheading:20722389-Protein Precursors,
pubmed-meshheading:20722389-Spectrometry, Mass, Matrix-Assisted Laser...
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pubmed:year |
2010
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pubmed:articleTitle |
Trichohyalin is a potential major autoantigen in human alopecia areata.
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pubmed:affiliation |
Centre for Skin Sciences, School of Life Sciences, University of Bradford, Bradford, West Yorkshire, United Kingdom.
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pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
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