Linked Life Data

20719268

Source:http://linkedlifedata.com/resource/pubmed/id/20719268

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:dateCreated
2010-8-19
pubmed:abstractText
Neuronal microtubules recently emerged as temporal and spatial regulators of dendritic spines, the major sites of excitatory synaptic input. By imaging microtubules in cultured mature primary hippocampal neurons using fluorescently tagged tubulin and microtubule plus-end binding (EB) protein EB3, dynamic microtubules were found to regularly depart from the dendritic shaft and enter dendritic spines. Evidence indicates that microtubule invasions into spines regulate spine actin dynamics and induce transient morphological changes, such as the formation of spine head protrusion and spine growth. Because alterations in spine morphology play an important role in synaptic plasticity and have been linked to learning and memory formation, it is possible that dynamic microtubules are engaged in adaptive processes in the adult brain. This chapter provides detailed methods for live imaging of dynamic microtubules in mature hippocampal neurons in culture. We describe protocols for culturing and transfecting mature hippocampal neurons and visualizing microtubules and microtubule plus-EB proteins by total internal reflection fluorescence microscopy and spinning disk confocal microscopy.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:status
MEDLINE
pubmed:issn
0091-679X
pubmed:author
pubmed:copyrightInfo
2010 Elsevier Inc. All rights reserved.
pubmed:issnType
Print
pubmed:volume
97
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
111-32
pubmed:meshHeading
pubmed:year
2010
pubmed:articleTitle
Microtubule dynamics in dendritic spines.
pubmed:affiliation
Department of Neuroscience, Erasmus Medical Center, 3015 GE, Rotterdam, The Netherlands.
pubmed:publicationType
Journal Article, Review, Research Support, Non-U.S. Gov't