Linked Life Data

20719060

Source:http://linkedlifedata.com/resource/pubmed/id/20719060

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
8
pubmed:dateCreated
2010-8-19
pubmed:abstractText
4-Aminophenol was selected as a model hapten for thermal-lens detection in a heterogeneous indirect immunoassay for small organic compounds. The assay is based on the competition of the free hapten and 4-aminophenol labeled with tetramethyl rhodamine isothiocyanate (TRITC) for rabbit anti-4-aminophenol antibodies (4-AP-GA-BSA) immobilized on a transparent poly(ethylene terephthalate) plate. The amount of the colored analyte was directly measured by thermal lensing at the plate surface. The developed method is specific for 4-aminophenol. The limit of detection is 2 x 10(-7) M, which corresponds to the absolute amount of 3 x 10(-11) mol of 4-aminophenol. The sensitivities of the developed thermal-lens and fluorescence-based measurements of the immunoassay under the same conditions are in good agreement with the expected and discussed theoretical sensitivities of photothermal and fluorescence measurements.
pubmed:language
eng
pubmed:journal
pubmed:status
PubMed-not-MEDLINE
pubmed:month
Aug
pubmed:issn
1943-3530
pubmed:author
pubmed:issnType
Electronic
pubmed:volume
64
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
942-8
pubmed:year
2010
pubmed:articleTitle
Heterogeneous thermal-lens immunoassay for small organic compounds: determination of 4-aminophenol.
pubmed:affiliation
Chemistry Department, M.V. Lomonosov Moscow State University, Leninskie Gory, 119991, GSP-1 Moscow, Russia.
pubmed:publicationType
Journal Article