Linked Life Data

20715996

Source:http://linkedlifedata.com/resource/pubmed/id/20715996

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
4
pubmed:dateCreated
2010-8-18
pubmed:abstractText
The beta8 integrin, which forms alphavbeta8 heterodimers, is being widely investigated because of its specific structure and functions compared with other integrins. In this report, a 12 aa-long peptide of beta8 integrin cytoplasmic domain was synthesized according to a published sequence and covalently coupled to keyhole limpet hemocyanin (KLH). Three stable strains of hybridomas (3G6, 5C7, 5H3) that can secrete high specific monoclonal antibodies against beta8 integrin were successfully established by hybridoma technique. The isotypes of these MAbs were tested to be IgG2a. Their characterizations were shown by enzyme-linked immunosorbent assay (ELISA), Western blot analysis, and immunocytochemistry (ICC). The affinity constants (Kaff ) of the MAbs 3G6, 5C7, and 5H3 were measured by non-competitive ELISA respectively. Western blot analyses and immunocytochemistry demonstrated that all the MAbs were directed against beta8 integrin with high specificity.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Aug
pubmed:issn
1557-8348
pubmed:author
pubmed:issnType
Electronic
pubmed:volume
29
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
361-6
pubmed:meshHeading
pubmed:year
2010
pubmed:articleTitle
Synthetic peptide coupled to KLH elicits antibodies against beta8 integrin.
pubmed:affiliation
Department of Immunology, School of Preclinical and Forensic Medicine, West China Medical Centre, Sichuan University, Chengdu, China.
pubmed:publicationType
Journal Article, Research Support, Non-U.S. Gov't