Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
20
pubmed:dateCreated
2010-9-29
pubmed:abstractText
CodY, a global regulatory protein that monitors the nutrient sufficiency of the environment by responding to the intracellular levels of GTP and the branched-chain amino acids, was previously shown to be a potent repressor of toxin gene expression in Clostridium difficile during growth in rich medium. In the intestinal tract, such derepression of toxin synthesis would lead to destruction of epithelial cells and the liberation of potential nutrients for the bacterium. CodY is likely to play an important role in regulating overall cellular physiology as well. In this study, DNA microarray analysis and affinity purification of CodY-DNA complexes were used to identify and distinguish the direct and indirect effects of CodY on global gene transcription. A codY null mutation resulted in >4-fold overexpression of 146 genes (organized in 82 apparent transcription units) and underexpression of 19 genes. In addition to the toxin genes, genes for amino acid biosynthesis, nutrient transport, fermentation pathways, membrane components, and surface proteins were overexpressed in the codY mutant. Genome-wide analysis identified more than 350 CodY binding regions, many of which are likely to correspond to sites of direct CodY-mediated regulation. About 60% of the CodY-repressed transcription units were associated with binding regions. Several of these genes were confirmed to be direct targets of CodY by gel mobility shift and DNase I footprinting assays.
pubmed:grant
pubmed:commentsCorrections
http://linkedlifedata.com/resource/pubmed/commentcorrection/20709897-11320220, http://linkedlifedata.com/resource/pubmed/commentcorrection/20709897-11331605, http://linkedlifedata.com/resource/pubmed/commentcorrection/20709897-11752295, http://linkedlifedata.com/resource/pubmed/commentcorrection/20709897-12100558, http://linkedlifedata.com/resource/pubmed/commentcorrection/20709897-12374831, http://linkedlifedata.com/resource/pubmed/commentcorrection/20709897-12618455, http://linkedlifedata.com/resource/pubmed/commentcorrection/20709897-13475269, http://linkedlifedata.com/resource/pubmed/commentcorrection/20709897-15228537, http://linkedlifedata.com/resource/pubmed/commentcorrection/20709897-15228538, http://linkedlifedata.com/resource/pubmed/commentcorrection/20709897-15802253, http://linkedlifedata.com/resource/pubmed/commentcorrection/20709897-15916606, http://linkedlifedata.com/resource/pubmed/commentcorrection/20709897-15937175, http://linkedlifedata.com/resource/pubmed/commentcorrection/20709897-16040604, http://linkedlifedata.com/resource/pubmed/commentcorrection/20709897-16322603, http://linkedlifedata.com/resource/pubmed/commentcorrection/20709897-16339935, http://linkedlifedata.com/resource/pubmed/commentcorrection/20709897-16488888, http://linkedlifedata.com/resource/pubmed/commentcorrection/20709897-16530465, http://linkedlifedata.com/resource/pubmed/commentcorrection/20709897-16923907, http://linkedlifedata.com/resource/pubmed/commentcorrection/20709897-16925561, http://linkedlifedata.com/resource/pubmed/commentcorrection/20709897-16995897, http://linkedlifedata.com/resource/pubmed/commentcorrection/20709897-17015669, http://linkedlifedata.com/resource/pubmed/commentcorrection/20709897-17302820, http://linkedlifedata.com/resource/pubmed/commentcorrection/20709897-17480182, http://linkedlifedata.com/resource/pubmed/commentcorrection/20709897-17510253, http://linkedlifedata.com/resource/pubmed/commentcorrection/20709897-17725558, http://linkedlifedata.com/resource/pubmed/commentcorrection/20709897-1779832, http://linkedlifedata.com/resource/pubmed/commentcorrection/20709897-17982469, http://linkedlifedata.com/resource/pubmed/commentcorrection/20709897-18024519, http://linkedlifedata.com/resource/pubmed/commentcorrection/20709897-18083814, http://linkedlifedata.com/resource/pubmed/commentcorrection/20709897-18156263, http://linkedlifedata.com/resource/pubmed/commentcorrection/20709897-18214442, http://linkedlifedata.com/resource/pubmed/commentcorrection/20709897-18539745, http://linkedlifedata.com/resource/pubmed/commentcorrection/20709897-18546601, http://linkedlifedata.com/resource/pubmed/commentcorrection/20709897-18714091, http://linkedlifedata.com/resource/pubmed/commentcorrection/20709897-18971494, http://linkedlifedata.com/resource/pubmed/commentcorrection/20709897-19251851, http://linkedlifedata.com/resource/pubmed/commentcorrection/20709897-19252482, http://linkedlifedata.com/resource/pubmed/commentcorrection/20709897-19651859, http://linkedlifedata.com/resource/pubmed/commentcorrection/20709897-19834554, http://linkedlifedata.com/resource/pubmed/commentcorrection/20709897-19844228, http://linkedlifedata.com/resource/pubmed/commentcorrection/20709897-20363936, http://linkedlifedata.com/resource/pubmed/commentcorrection/20709897-6870225, http://linkedlifedata.com/resource/pubmed/commentcorrection/20709897-7584402, http://linkedlifedata.com/resource/pubmed/commentcorrection/20709897-7775453, http://linkedlifedata.com/resource/pubmed/commentcorrection/20709897-7777059, http://linkedlifedata.com/resource/pubmed/commentcorrection/20709897-8899962, http://linkedlifedata.com/resource/pubmed/commentcorrection/20709897-8973304, http://linkedlifedata.com/resource/pubmed/commentcorrection/20709897-9075217, http://linkedlifedata.com/resource/pubmed/commentcorrection/20709897-9108241, http://linkedlifedata.com/resource/pubmed/commentcorrection/20709897-9466260, http://linkedlifedata.com/resource/pubmed/commentcorrection/20709897-9509270, http://linkedlifedata.com/resource/pubmed/commentcorrection/20709897-9514706
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Oct
pubmed:issn
1098-5530
pubmed:author
pubmed:issnType
Electronic
pubmed:volume
192
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
5350-62
pubmed:dateRevised
2011-7-27
pubmed:meshHeading
pubmed:year
2010
pubmed:articleTitle
Integration of metabolism and virulence by Clostridium difficile CodY.
pubmed:affiliation
Department of Molecular Biology and Microbiology, Tufts University School of Medicine, Boston, Massachusetts 02111, USA.
pubmed:publicationType
Journal Article, Research Support, N.I.H., Extramural