Linked Life Data

20708077

Source:http://linkedlifedata.com/resource/pubmed/id/20708077

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
3
pubmed:dateCreated
2010-11-24
pubmed:abstractText
Size is the main protein characteristic that determines its release from non-degrading neutral hydrogels. The effect of network charge on the release of proteins has not been studied systematically so far. Therefore, we investigated the release of proteins from charged hydrogels that were obtained by co-polymerization of methacrylated dextran (Dex-MA) with either methacrylic acid (MA) or 2-N,N-dimethylaminoethyl methacrylate (DMAEMA). These hydrogels are stable under physiological conditions. The effect of incorporation of the charged monomers on hydrogel charge, equilibrium swelling, and release of model proteins was assessed at both low (10mM HEPES) and physiological ionic strength (HEPES buffered saline, HBS). Model proteins were chosen on the basis of their charge at physiological pH; bovine serum albumin (BSA, negatively charged), myoglobin (neutral), and cytochrome C (positively charged). Interestingly, as opposed to myoglobin, both charged proteins were fully immobilized in the networks with opposite charge by electrostatic interaction at low ionic strength. On the other hand, at physiological ionic strength, the percentage of immobilized protein depended on the charge density of the hydrogel. For all proteins, the diffusion coefficient of the mobile fractions was not affected by opposite network charge. However, the release rate of BSA from similarly (negatively) charged networks significantly increased when a relatively high amount of charged monomers was incorporated. We conclude that incorporation of charge in a hydrogel network is suited as a tool for the immobilization of proteins and triggered release by increasing ionic strength.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Nov
pubmed:issn
1873-3441
pubmed:author
pubmed:copyrightInfo
Copyright © 2010 Elsevier B.V. All rights reserved.
pubmed:issnType
Electronic
pubmed:volume
76
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
329-35
pubmed:meshHeading
pubmed:year
2010
pubmed:articleTitle
The effect of network charge on the immobilization and release of proteins from chemically crosslinked dextran hydrogels.
pubmed:affiliation
Department of Pharmaceutics, Utrecht University, Utrecht, The Netherlands.
pubmed:publicationType
Journal Article, Research Support, Non-U.S. Gov't