Linked Life Data

2070796

Source:http://linkedlifedata.com/resource/pubmed/id/2070796

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
2
pubmed:dateCreated
1991-8-20
pubmed:databankReference
pubmed:abstractText
We have isolated cDNAs encoding the alpha 6 subunit from a lambda gt11 expression library from human keratinocytes by combined screening with a rabbit polyclonal anti-alpha 6 antibody and the polymerase chain reaction. The alpha 6 subunit encoded by this cDNA consists of 1050 amino acids with a 991-amino-acid extracellular, a 23-amino-acid transmembrane and a 36-amino-acid cytoplasmic domain. The extracellular domain contains three putative divalent cation-binding sites and nine potential N-linked glycosylation sites. From a cDNA library from normal human mammary gland cells two different cDNAs for alpha 6 were isolated, one of which is identical to the above cDNA. The two alpha 6 subunits, called alpha 6A and alpha 6B, encoded by the two cDNAs each have a unique cytoplasmic domain, that of alpha 6B being 18 amino acids longer than that of alpha 6A. Different carcinoma cell lines contain transcripts for both alpha 6 subunits. K562 leukemic cells have little alpha 6A or alpha 6B mRNAs. The overall level of expression varies in the carcinoma cell lines, but reflects alpha 6 cell surface expression. In A375 melanoma cells, however, cell surface expression of alpha 6 was low in spite of a high level of mRNA. This suggest that other mechanisms may be involved in regulating the expression of alpha 6 on the surface of these cells. The mRNA for both alpha 6 subunits is around 6 kb. The alpha 6 subunits are similar to other alpha subunits (26-31% identity with cleaved alpha subunits) of the integrin family but they are more similar to the alpha 3 subunit (40% identity). This high degree of similarity may be the basis for their functional resemblance since both alpha 3 and alpha 6 subunits, when associated with beta 1, function as laminin receptors and bind to the long arm of laminin. The genes for alpha 6 and beta 4, the alternative beta subunit with which alpha 6 combines on certain epithelial cells, were mapped to chromosome 2 and 17q11-qter, respectively.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Jul
pubmed:issn
0014-2956
pubmed:author
pubmed:issnType
Print
pubmed:day
15
pubmed:volume
199
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
425-33
pubmed:dateRevised
2007-7-23
pubmed:meshHeading
pubmed-meshheading:2070796-Amino Acid Sequence, pubmed-meshheading:2070796-Base Sequence, pubmed-meshheading:2070796-Cell Line, pubmed-meshheading:2070796-Chromosome Banding, pubmed-meshheading:2070796-Chromosome Mapping, pubmed-meshheading:2070796-Chromosomes, Human, Pair 17, pubmed-meshheading:2070796-Chromosomes, Human, Pair 2, pubmed-meshheading:2070796-Cloning, Molecular, pubmed-meshheading:2070796-Gene Library, pubmed-meshheading:2070796-Humans, pubmed-meshheading:2070796-Integrins, pubmed-meshheading:2070796-Keratinocytes, pubmed-meshheading:2070796-Macromolecular Substances, pubmed-meshheading:2070796-Molecular Sequence Data, pubmed-meshheading:2070796-Oligonucleotide Probes, pubmed-meshheading:2070796-Polymerase Chain Reaction, pubmed-meshheading:2070796-RNA, Messenger, pubmed-meshheading:2070796-RNA Splicing, pubmed-meshheading:2070796-Restriction Mapping, pubmed-meshheading:2070796-Sequence Homology, Nucleic Acid
pubmed:year
1991
pubmed:articleTitle
Molecular cloning of the human alpha 6 integrin subunit. Alternative splicing of alpha 6 mRNA and chromosomal localization of the alpha 6 and beta 4 genes.
pubmed:affiliation
Central Laboratory of the Netherlands Red Cross Blood Transfusion Service, Amsterdam.
pubmed:publicationType
Journal Article, Comparative Study, Research Support, Non-U.S. Gov't