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rdf:type |
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lifeskim:mentions |
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pubmed:dateCreated |
2010-8-13
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pubmed:abstractText |
Cell proliferation is the main driving force for plant growth. Although genome sequence analysis revealed a high number of cell cycle genes in plants, little is known about the molecular complexes steering cell division. In a targeted proteomics approach, we mapped the core complex machinery at the heart of the Arabidopsis thaliana cell cycle control. Besides a central regulatory network of core complexes, we distinguished a peripheral network that links the core machinery to up- and downstream pathways. Over 100 new candidate cell cycle proteins were predicted and an in-depth biological interpretation demonstrated the hypothesis-generating power of the interaction data. The data set provided a comprehensive view on heterodimeric cyclin-dependent kinase (CDK)-cyclin complexes in plants. For the first time, inhibitory proteins of plant-specific B-type CDKs were discovered and the anaphase-promoting complex was characterized and extended. Important conclusions were that mitotic A- and B-type cyclins form complexes with the plant-specific B-type CDKs and not with CDKA;1, and that D-type cyclins and S-phase-specific A-type cyclins seem to be associated exclusively with CDKA;1. Furthermore, we could show that plants have evolved a combinatorial toolkit consisting of at least 92 different CDK-cyclin complex variants, which strongly underscores the functional diversification among the large family of cyclins and reflects the pivotal role of cell cycle regulation in the developmental plasticity of plants.
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
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pubmed:status |
MEDLINE
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pubmed:month |
Aug
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pubmed:issn |
1744-4292
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pubmed:author |
pubmed-author:BeynonJimJ,
pubmed-author:BuffelYelleY,
pubmed-author:De BodtStefanieS,
pubmed-author:De JaegerGeertG,
pubmed-author:De VeylderLievenL,
pubmed-author:EeckhoutDominiqueD,
pubmed-author:EloyNubiaN,
pubmed-author:FaureJean-DenisJD,
pubmed-author:FerreiraPaulo C GPC,
pubmed-author:HilsonPierreP,
pubmed-author:HollunderJensJ,
pubmed-author:InzéDirkD,
pubmed-author:KuiperMartinM,
pubmed-author:LarkinJohn CJC,
pubmed-author:LaukensKrisK,
pubmed-author:MaereStevenS,
pubmed-author:MeyerChristianC,
pubmed-author:NeirynckSandyS,
pubmed-author:PersiauGeertG,
pubmed-author:PharazynAnneA,
pubmed-author:RenneCharlotteC,
pubmed-author:StalsHildeH,
pubmed-author:SteinbrennerJensJ,
pubmed-author:Van De SlijkeEvelineE,
pubmed-author:Van IsterdaelGertG,
pubmed-author:Van LeeneJelleJ,
pubmed-author:Van OnckelenHarryH,
pubmed-author:Van de PeerYvesY,
pubmed-author:VerkestAurineA,
pubmed-author:WittersErwinE
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pubmed:issnType |
Electronic
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pubmed:day |
10
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pubmed:volume |
6
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
397
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pubmed:meshHeading |
pubmed-meshheading:20706207-Arabidopsis,
pubmed-meshheading:20706207-Arabidopsis Proteins,
pubmed-meshheading:20706207-Cell Cycle,
pubmed-meshheading:20706207-Cell Cycle Proteins,
pubmed-meshheading:20706207-Computational Biology,
pubmed-meshheading:20706207-Cyclin-Dependent Kinases,
pubmed-meshheading:20706207-Cyclins,
pubmed-meshheading:20706207-DNA Replication,
pubmed-meshheading:20706207-Luciferases,
pubmed-meshheading:20706207-Mitosis,
pubmed-meshheading:20706207-Models, Biological,
pubmed-meshheading:20706207-Multiprotein Complexes,
pubmed-meshheading:20706207-Protein Binding,
pubmed-meshheading:20706207-Protein Interaction Mapping,
pubmed-meshheading:20706207-Reproducibility of Results
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pubmed:year |
2010
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pubmed:articleTitle |
Targeted interactomics reveals a complex core cell cycle machinery in Arabidopsis thaliana.
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pubmed:affiliation |
Department of Plant Systems Biology, VIB, Ghent, Belgium.
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pubmed:publicationType |
Journal Article,
Research Support, U.S. Gov't, Non-P.H.S.,
Research Support, Non-U.S. Gov't
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