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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
22
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pubmed:dateCreated |
2010-12-14
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pubmed:abstractText |
eIF4E-binding proteins (4E-BPs) regulate translation of mRNAs in eukaryotes. However the extent to which specific mRNA targets are regulated by 4E-BPs remains unknown. We performed translational profiling by microarray analysis of polysome and monosome associated mRNAs in wild-type and mutant cells to identify mRNAs in yeast regulated by the 4E-BPs Caf20p and Eap1p; the first-global comparison of 4E-BP target mRNAs. We find that yeast 4E-BPs modulate the translation of >1000 genes. Most target mRNAs differ between the 4E-BPs revealing mRNA specificity for translational control by each 4E-BP. This is supported by observations that eap1? and caf20? cells have different nitrogen source utilization defects, implying different mRNA targets. To account for the mRNA specificity shown by each 4E-BP, we found correlations between our data sets and previously determined targets of yeast mRNA-binding proteins. We used affinity chromatography experiments to uncover specific RNA-stabilized complexes formed between Caf20p and Puf4p/Puf5p and between Eap1p and Puf1p/Puf2p. Thus the combined action of each 4E-BP with specific 3'-UTR-binding proteins mediates mRNA-specific translational control in yeast, showing that this form of translational control is more widely employed than previously thought.
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pubmed:grant |
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
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pubmed:status |
MEDLINE
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pubmed:month |
Dec
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pubmed:issn |
1362-4962
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pubmed:author |
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pubmed:issnType |
Electronic
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pubmed:volume |
38
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
8039-50
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pubmed:dateRevised |
2011-8-24
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pubmed:meshHeading |
pubmed-meshheading:20705650-Amino Acids,
pubmed-meshheading:20705650-Cyclins,
pubmed-meshheading:20705650-Eukaryotic Initiation Factors,
pubmed-meshheading:20705650-Gene Deletion,
pubmed-meshheading:20705650-Gene Expression Regulation, Fungal,
pubmed-meshheading:20705650-Nitrogen,
pubmed-meshheading:20705650-Oligonucleotide Array Sequence Analysis,
pubmed-meshheading:20705650-Protein Biosynthesis,
pubmed-meshheading:20705650-RNA, Messenger,
pubmed-meshheading:20705650-RNA-Binding Proteins,
pubmed-meshheading:20705650-Saccharomyces cerevisiae,
pubmed-meshheading:20705650-Saccharomyces cerevisiae Proteins,
pubmed-meshheading:20705650-Transcription, Genetic,
pubmed-meshheading:20705650-Transcription Factors
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pubmed:year |
2010
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pubmed:articleTitle |
Identifying eIF4E-binding protein translationally-controlled transcripts reveals links to mRNAs bound by specific PUF proteins.
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pubmed:affiliation |
The Michael Smith Building, Faculty of Life Sciences, University of Manchester, Manchester, UK.
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pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
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