Source:http://linkedlifedata.com/resource/pubmed/id/20703102
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| Predicate | Object |
|---|---|
| rdf:type | |
| lifeskim:mentions | |
| pubmed:issue |
8
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| pubmed:dateCreated |
2011-3-1
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| pubmed:abstractText |
The microRNA-17-92 cluster is an oncogene in human B cell lymphomas and lung cancers. Previous microRNA microarray data revealed that miR-17-5p, a member of the miR-17-92 cluster, is upregulated in pancreatic cancer. However, the involvement of miR-17-5p expression in pancreatic carcinogenesis has not well been studied. In the present study, we measured the miR-17-5p expression levels in pancreatic cancer cell lines, primary cultures of normal human pancreatic ductal cells, formalin-fixed paraffin-embedded (FFPE) tissue samples derived from 80 patients who underwent pancreatectomy for pancreatic cancer and microdissected cells (including normal ductal epithelial, pancreatic intraepithelial neoplasia-1B and invasive ductal carcinoma cells) by qRT-PCR. Furthermore, we investigated the effects of upregulation of miR-17-5p expression on the proliferation and invasion of pancreatic cancer cells. We found that pancreatic cancer cells expressed higher levels of miR-17-5p than primary cultured normal ductal cells. miR-17-5p was also overexpressed in pancreatic cancer in FFPE and microdissected samples. Furthermore, analysis of macrodissected FFPE samples revealed that high miR-17-5p expression was associated with a poor prognosis (p = 0.03). In addition, in vitro experiments revealed that SUIT-2 and KP-2 pancreatic cancer cells transfected with the miR-17-5p precursor showed significantly higher cell growth ratios than the corresponding control cells (p < 0.001 and p = 0.012, respectively), as well as significantly higher numbers of invading cells (p < 0.0001 for both). The present findings suggest that miR-17-5p plays important roles in pancreatic carcinogenesis and cancer progression, and is associated with a poor prognosis in pancreatic cancer.
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| pubmed:language |
eng
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| pubmed:journal | |
| pubmed:citationSubset |
IM
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| pubmed:chemical | |
| pubmed:status |
MEDLINE
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| pubmed:month |
Oct
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| pubmed:issn |
1555-8576
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| pubmed:author | |
| pubmed:issnType |
Electronic
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| pubmed:day |
15
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| pubmed:volume |
10
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| pubmed:owner |
NLM
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| pubmed:authorsComplete |
Y
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| pubmed:pagination |
748-57
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| pubmed:meshHeading |
pubmed-meshheading:20703102-Adult,
pubmed-meshheading:20703102-Aged,
pubmed-meshheading:20703102-Aged, 80 and over,
pubmed-meshheading:20703102-Cell Line, Tumor,
pubmed-meshheading:20703102-Cell Proliferation,
pubmed-meshheading:20703102-Female,
pubmed-meshheading:20703102-Gene Expression Regulation, Neoplastic,
pubmed-meshheading:20703102-Humans,
pubmed-meshheading:20703102-Male,
pubmed-meshheading:20703102-MicroRNAs,
pubmed-meshheading:20703102-Middle Aged,
pubmed-meshheading:20703102-Multivariate Analysis,
pubmed-meshheading:20703102-Neoplasm Invasiveness,
pubmed-meshheading:20703102-Pancreatic Neoplasms,
pubmed-meshheading:20703102-Paraffin Embedding,
pubmed-meshheading:20703102-Prognosis,
pubmed-meshheading:20703102-Reverse Transcriptase Polymerase Chain Reaction,
pubmed-meshheading:20703102-Survival Analysis,
pubmed-meshheading:20703102-Tissue Fixation
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| pubmed:year |
2010
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| pubmed:articleTitle |
MicroRNA miR-17-5p is overexpressed in pancreatic cancer, associated with a poor prognosis, and involved in cancer cell proliferation and invasion.
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| pubmed:affiliation |
Department of Surgery and Oncology, Graduate School of Medical Sciences, Kyushu University, Fukuoka, Japan.
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| pubmed:publicationType |
Journal Article
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