Linked Life Data

20698509

Source:http://linkedlifedata.com/resource/pubmed/id/20698509

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
17
pubmed:dateCreated
2010-9-2
pubmed:abstractText
Anthracyclines are powerful chemotherapeutic agents for the treatment of many cancers. In many instances, they are currently used in combination with histone deacetylase inhibitors in order to enhance their efficiency. Not surprisingly and as part of their mode of action, these drugs interfere with gene expression, a process that has long been known to be mediated by histone acetylation. In this paper, we use analytical ultracentrifuge analysis, equilibrium dialysis, and circular dichroism to characterize the role of histone acetylation on the binding of antharcyclines to chromatin. We show that histone acetylation enhances the daunomycin-induced DNA dissociation from nucleosomes and decreases the extent of aggregation that results from the interaction in a way that is modulated by the presence or absence of linker histones. Histone acetylation increases the binding affinity of daunomycin by chromatin. Furthermore, the binding of anthracycline to acetylated chromatin sheds additional light into the conformational chromatin alterations resulting from core histone acetylation.
pubmed:grant
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Sep
pubmed:issn
1520-4804
pubmed:author
pubmed:issnType
Electronic
pubmed:day
9
pubmed:volume
53
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
6457-65
pubmed:meshHeading
pubmed:year
2010
pubmed:articleTitle
Interaction of daunomycin with acetylated chromatin.
pubmed:affiliation
Department of Biochemistry and Microbiology, University of Victoria, Victoria, British Columbia, V8W 3P6, Canada.
pubmed:publicationType
Journal Article, Research Support, Non-U.S. Gov't