Linked Life Data

20695900

Source:http://linkedlifedata.com/resource/pubmed/id/20695900

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
2
pubmed:dateCreated
2010-8-24
pubmed:abstractText
TraJ is an activator of the transfer (tra) operon in the F plasmid that counteracts H-NS silencing at the main transfer promoter (P(Y)). TraJ contains 226 aa (26 670 kDa), not 229 aa as reported previously, and forms homodimers. TraJ binds DNA containing P(Y)in vivo as demonstrated using a chromatin-immunoprecipitation assay. Mutations within a predicted helix-turn-helix DNA-binding motif reduced binding and decreased mating efficiency. The deletion of four or more residues from the C-terminus of TraJ blocked its activity, but did not interfere with DNA binding. This feature, as well as homology to the C-terminal region of RovA and SlyA within the MarR/SlyA family, suggests that TraJ might counteract H-NS repression via a mechanism similar to these desilencing proteins.
pubmed:grant
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Sep
pubmed:issn
1574-6968
pubmed:author
pubmed:issnType
Electronic
pubmed:day
1
pubmed:volume
310
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
112-9
pubmed:meshHeading
pubmed:year
2010
pubmed:articleTitle
The F plasmid transfer activator TraJ is a dimeric helix-turn-helix DNA-binding protein.
pubmed:affiliation
Department of Biological Sciences, University of Alberta, Edmonton, Alberta, Canada.
pubmed:publicationType
Journal Article, Research Support, Non-U.S. Gov't