Linked Life Data

20694853

Source:http://linkedlifedata.com/resource/pubmed/id/20694853

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
4
pubmed:dateCreated
2011-3-22
pubmed:abstractText
The effect of trace metal ions (Co²+, Cu²+, Fe²+, Mn²+, Mo?+, Ni²+, Zn²+, SeO?? and WO??) on growth and ethanol production by an ethanologenic acetogen, Clostridium ragsdalei was investigated in CO:CO?-grown cells. A standard acetogen medium (ATCC medium no. 1754) was manipulated by varying the concentrations of trace metals in the media. Increasing the individual concentrations of Ni²+, Zn²+, SeO?? and WO?? from 0.84, 6.96, 1.06, and 0.68 ?M in the standard trace metals solution to 8.4, 34.8, 5.3, and 6.8 ?M, respectively, increased ethanol production from 35.73 mM under standard metals concentration to 176.5, 187.8, 54.4, and 72.3 mM, respectively. Nickel was necessary for growth of C. ragsdalei. Growth rate (?) of C. ragsdalei improved from 0.34 to 0.49 (day?¹), and carbon monoxide dehydrogenase (CODH) and hydrogenase (H?ase)-specific activities improved from 38.45 and 0.35 to 48.5 and 1.66 U/mg protein, respectively, at optimum concentration of Ni²+. At optimum concentrations of WO?? and SeO??, formate dehydrogenase (FDH) activity improved from 32.3 to 42.6 and 45.4 U/mg protein, respectively. Ethanol production and the activity of FDH reduced from 35 mM and 32.3 U/mg protein to 1.14 mM and 8.79 U/mg protein, respectively, upon elimination of WO?? from the medium. Although increased concentration of Zn²+ enhanced growth and ethanol production, the activities of CODH, FDH, H?ase and alcohol dehydrogenase (ADH) were not affected by varying the Zn²+ concentration. Omitting Fe²+ from the medium decreased ethanol production from 35.7 to 6.30 mM and decreased activities of CODH, FDH, H?ase and ADH from 38.5, 32.3, 0.35, and 0.68 U/mg protein to 9.07, 7.01, 0.10, and 0.24 U/mg protein, respectively. Ethanol production improved from 35 to 54 mM when Cu²+ was removed from the medium. The optimization of trace metals concentration in the fermentation medium improved enzyme activities (CODH, FDH, and H?ase), growth and ethanol production by C. ragsdalei.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Apr
pubmed:issn
1476-5535
pubmed:author
pubmed:issnType
Electronic
pubmed:volume
38
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
513-21
pubmed:meshHeading
pubmed:year
2011
pubmed:articleTitle
Effect of trace metals on ethanol production from synthesis gas by the ethanologenic acetogen, Clostridium ragsdalei.
pubmed:affiliation
Department of Botany and Microbiology, University of Oklahoma, Norman, OK 73019, USA. Jsaxena@coskata.com
pubmed:publicationType
Journal Article, Research Support, U.S. Gov't, Non-P.H.S.