Source:http://linkedlifedata.com/resource/pubmed/id/20692235
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Predicate | Object |
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rdf:type | |
lifeskim:mentions | |
pubmed:issue |
1
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pubmed:dateCreated |
2010-9-13
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pubmed:abstractText |
Autotaxin, encoded by the Enpp2 gene, generates lysophosphatidic acid (LPA) extracellularly, eliciting various cellular responses through specific LPA receptors. Previous studies have revealed that Enpp2(-/-) mice die at E9.5 owing to angiogenic defects in the yolk sac. Moreover, Enpp2(-/-) embryos show growth retardation, allantois malformation, no axial turning, and head cavity formation. We have also demonstrated that lysosome biogenesis is impaired in yolk sac visceral endoderm cells of Enpp2(-/-) embryos as a result of the downregulation of the Rho-ROCK (Rho-associated coiled-coil containing protein kinase)-LIM kinase pathway. In this study, we examine what signaling defect(s) is responsible for head cavity formation and yolk sac angiogenic defects. By using a whole embryo culture system, we show that 10 ?M Ki16425, an antagonist for the LPA receptors, induces head cavity formation and yolk sac angiogenic defects in wild-type embryos. Moreover, 1 ?M Ki16425 induces both phenotypes in Enpp2 heterozygous embryos at significantly higher incidence than in wild-type embryos, suggesting an interaction between autotaxin and LPA receptor signaling. Furthermore, we show that inhibition of the Rho-ROCK pathway induces head cavity formation, whereas multiple pathways are involved in yolk sac angiogenic defects. These results reveal the signal transduction defects that underlie the abnormalities in Enpp2(-/-) embryos.
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pubmed:language |
eng
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pubmed:journal | |
pubmed:citationSubset |
IM
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pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/Actins,
http://linkedlifedata.com/resource/pubmed/chemical/Multienzyme Complexes,
http://linkedlifedata.com/resource/pubmed/chemical/Phosphodiesterase I,
http://linkedlifedata.com/resource/pubmed/chemical/Phosphoric Diester Hydrolases,
http://linkedlifedata.com/resource/pubmed/chemical/Pyrophosphatases,
http://linkedlifedata.com/resource/pubmed/chemical/Receptors, Lysophosphatidic Acid,
http://linkedlifedata.com/resource/pubmed/chemical/Rho Factor,
http://linkedlifedata.com/resource/pubmed/chemical/alkylglycerophosphoethanolamine...,
http://linkedlifedata.com/resource/pubmed/chemical/rho-Associated Kinases
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pubmed:status |
MEDLINE
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pubmed:month |
Sep
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pubmed:issn |
1090-2104
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pubmed:author | |
pubmed:copyrightInfo |
Copyright © 2010 Elsevier Inc. All rights reserved.
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pubmed:issnType |
Electronic
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pubmed:day |
10
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pubmed:volume |
400
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
66-71
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pubmed:dateRevised |
2011-11-7
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pubmed:meshHeading |
pubmed-meshheading:20692235-Actins,
pubmed-meshheading:20692235-Animals,
pubmed-meshheading:20692235-Blood Vessels,
pubmed-meshheading:20692235-Embryo, Mammalian,
pubmed-meshheading:20692235-Head,
pubmed-meshheading:20692235-Mice,
pubmed-meshheading:20692235-Mice, Mutant Strains,
pubmed-meshheading:20692235-Multienzyme Complexes,
pubmed-meshheading:20692235-Neovascularization, Physiologic,
pubmed-meshheading:20692235-Phosphodiesterase I,
pubmed-meshheading:20692235-Phosphoric Diester Hydrolases,
pubmed-meshheading:20692235-Pyrophosphatases,
pubmed-meshheading:20692235-Receptors, Lysophosphatidic Acid,
pubmed-meshheading:20692235-Rho Factor,
pubmed-meshheading:20692235-Signal Transduction,
pubmed-meshheading:20692235-Yolk Sac,
pubmed-meshheading:20692235-rho-Associated Kinases
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pubmed:year |
2010
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pubmed:articleTitle |
Deficiency of autotaxin/lysophospholipase D results in head cavity formation in mouse embryos through the LPA receptor-Rho-ROCK pathway.
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pubmed:affiliation |
Department of Molecular Neurobiology, Graduate School of Comprehensive Human Sciences, Institute of Basic Medical Sciences, University of Tsukuba, 1-1-1 Tennodai, Tsukuba, Ibaraki 305-8577, Japan.
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pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
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