Linked Life Data

20691739

Source:http://linkedlifedata.com/resource/pubmed/id/20691739

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
3
pubmed:dateCreated
2010-11-8
pubmed:abstractText
Encapsulating delicate biomolecules into submicron-sized polymer particulate systems with preserved native conformation and sufficient loading efficiency is of great challenge. To address this issue, we developed a unique polymersome which differs from reported polymersome in that its bilayer membrane was formed of two different amphiphilic diblock copolymers in an "asymmetric" way. By adding two diblock copolymers, poly (ethylene glycol)-block-poly (?-caprolactone) (PEG-PCL) and dextran-block-poly (?-caprolactone) (DEX-PCL), into a so-called dextran-in-PEG aqueous two-phase system, DEX-PCL formed the inner leaflet around the dispersed dextran phase and PEG-PCL formed the outer leaflet with the PEG block facing the PEG continuous phase. We name this unique assembly process as "phase-guided assembly". Polymersomes of asymmetric bilayer membrane possess a series of advantages over "symmetric" polymer bilayer vesicles previously reported. The asymmetric bilayer created a different chemical environment of the interior to which proteins were encapsulated highly efficiently (up to 90%) by thermodynamically favored partition. Probably due to the thermodynamic preference, erythropoietin (EPO) encapsulated in this system showed a well-preserved bioactivity in cell proliferation assay. The core of the polymersomes may be cross-linked to enhance their mechanical strength. Phase-guided assembly system and asymmetric bilayer polymersomes demonstrated in this study may serve the high demands for delivering nucleotide and protein medicines and other biological applications.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Nov
pubmed:issn
1873-4995
pubmed:author
pubmed:copyrightInfo
Copyright © 2010 Elsevier B.V. All rights reserved.
pubmed:issnType
Electronic
pubmed:day
1
pubmed:volume
147
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
413-9
pubmed:meshHeading
pubmed-meshheading:20691739-Cell Line, Tumor, pubmed-meshheading:20691739-Cell Proliferation, pubmed-meshheading:20691739-Chemistry, Pharmaceutical, pubmed-meshheading:20691739-Dextrans, pubmed-meshheading:20691739-Drug Carriers, pubmed-meshheading:20691739-Drug Compounding, pubmed-meshheading:20691739-Erythropoietin, pubmed-meshheading:20691739-Humans, pubmed-meshheading:20691739-Hydrophobic and Hydrophilic Interactions, pubmed-meshheading:20691739-Kinetics, pubmed-meshheading:20691739-Lactones, pubmed-meshheading:20691739-Liquid Crystals, pubmed-meshheading:20691739-Megakaryocytes, pubmed-meshheading:20691739-Membranes, Artificial, pubmed-meshheading:20691739-Microscopy, Confocal, pubmed-meshheading:20691739-Molecular Conformation, pubmed-meshheading:20691739-Polyesters, pubmed-meshheading:20691739-Polyethylene Glycols, pubmed-meshheading:20691739-Solubility, pubmed-meshheading:20691739-Technology, Pharmaceutical
pubmed:year
2010
pubmed:articleTitle
Polymersomes of asymmetric bilayer membrane formed by phase-guided assembly.
pubmed:affiliation
Laboratory of Drug Delivery and Biomaterials, School of Pharmacy, Shanghai Jiao Tong University, Shanghai 200240, PR China.
pubmed:publicationType
Journal Article, Research Support, Non-U.S. Gov't