Linked Life Data

20691733

Source:http://linkedlifedata.com/resource/pubmed/id/20691733

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
2
pubmed:dateCreated
2010-9-29
pubmed:abstractText
A primary therapeutic goal in Alzheimer's disease (AD) is to reduce the quantity of amyloid ? protein (A?) present in the brain. To develop an effective, safe system for vaccination against Alzheimer's disease, the plant virus Cucumber mosaic virus (CMV) was engineered genetically to express A?-derived fragments that stimulate mainly humoral immune responses. Six chimeric constructs, bearing the A?1-15 or the A?4-15 sequence in positions 248, 392 or 529 of the CMV coat protein (CP) gene, were created. Viral products proved to be able to replicate in their natural host. However, only chimeric A?1-15-CMVs were detected by A?1-42 antiserum in Western blot analysis. Experimental evidence of Immunoelectron microscopy revealed a complete decoration of A?1-15-CMV(248) and A?1-15-CMV(392) following incubation with either anti-A?1-15 or anti-A?1-42 polyclonal antibodies. These two chimeric CMVs appear to be endowed with features making them possible candidates for vaccination against Alzheimer's disease.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Nov
pubmed:issn
1879-0984
pubmed:author
pubmed:copyrightInfo
Copyright © 2010 Elsevier B.V. All rights reserved.
pubmed:issnType
Electronic
pubmed:volume
169
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
332-40
pubmed:meshHeading
pubmed:year
2010
pubmed:articleTitle
Cucumber mosaic virus as the expression system for a potential vaccine against Alzheimer's disease.
pubmed:affiliation
Department of Biology, Plant Protection and Agrobiotechnology, University of Basilicata, Viale dell'Ateneo Lucano, 10, 85100 Potenza, Italy.
pubmed:publicationType
Journal Article, Research Support, Non-U.S. Gov't