Linked Life Data

20691709

Source:http://linkedlifedata.com/resource/pubmed/id/20691709

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
1-2
pubmed:dateCreated
2010-10-4
pubmed:abstractText
The micronucleus (MN) test is widely used to biomonitor humans exposed to clastogens and aneugens, but little is known about MN development. Here we used confocal time-lapse imaging and a fluorescent human lymphoblastoid cell line (T105GTCH), in which histone H3 and ?-tubulin stained differentially, to record the emergence and behavior of micronuclei (MNi) in cells exposed to MN-inducing agents. In mitomycin C (MMC)-treated cells, MNi originated in early anaphase from lagging chromosome fragments just after chromosome segregation. In ?-ray-treated cells showing multipolar cell division, MN originated in late anaphase from lagging chromosome fragments generated by the abnormal cell division associated with supernumerary centrosomes. In vincristine(VC)-treated cells, MN formation was similar to that in MMC-treated cells, but MNi were also derived from whole chromosomes that did not align properly on the metaphase plate. Thus, the MN formation process induced by MMC, ?-rays, and VC, were strikingly different, suggesting that different mechanisms were involved. MN stability, however, was similar regardless of the treatment and unrelated to MN formation mechanisms. MNi were stable in daughter cells, and MN-harboring cells tended to die during cell cycle progression with greater frequency than cells without MN. Because of their persistence, MN may have significant impact on cells, causing genomic instability and abnormally transcribed genes.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Oct
pubmed:issn
0027-5107
pubmed:author
pubmed:copyrightInfo
Copyright © 2010 Elsevier B.V. All rights reserved.
pubmed:issnType
Print
pubmed:day
13
pubmed:volume
692
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
12-8
pubmed:meshHeading
pubmed:year
2010
pubmed:articleTitle
Live cell imaging of micronucleus formation and development.
pubmed:affiliation
Division of Genetics and Mutagenesis, National Institute of Health Sciences, Tokyo 158-8501, Japan. m-yasui@nihs.go.jp
pubmed:publicationType
Journal Article, Research Support, Non-U.S. Gov't