Source:http://linkedlifedata.com/resource/pubmed/id/20689058
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Predicate | Object |
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rdf:type | |
lifeskim:mentions | |
pubmed:issue |
4
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pubmed:dateCreated |
2010-9-29
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pubmed:abstractText |
Activated pancreatic stellate cells (PSCs) play a pivotal role in pancreatic fibrosis in chronic pancreatitis and pancreatic cancer. Recent studies have suggested a role of IL-33, a newly identified IL-1 family member, in fibrosis. We here examined the expression of IL-33 and the IL-33-mediated regulation of cell functions in PSCs. PSCs were isolated from human and rat pancreas tissues. The expression of IL-33 was examined by Western blotting, PCR, ELISA, and immunostaining. The roles of IL-33 in the regulation of PSC functions were examined by using recombinant IL-33 and small interfering RNA. Activated PSCs expressed IL-33 in the nucleus, and the expression was increased by IL-1?, TNF-?, PDGF-BB, and IFN-?, but not TGF-?1. Nuclear IL-33 expression was also observed in the pancreatic acinar and ductal cells. IL-1? induced IL-33 expression mainly through the activation of NF-?B and ERK pathways and partially through that of p38 MAP kinase, whereas PDGF-BB induced IL-33 expression mainly through the activation of ERK pathway. PSCs expressed soluble ST2, ST2L, and IL-1RAcP, but the expression level of ST2L was relatively low. Recombinant IL-33 did not stimulate key cell functions of PSCs. Decreased IL-33 expression by small interfering RNA resulted in decreased proliferation in response to PDGF-BB. In conclusion, activated PSCs expressed IL-33 in the nucleus. IL-33 might regulate the PDGF-induced proliferation in PSCs.
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pubmed:language |
eng
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pubmed:journal | |
pubmed:citationSubset |
IM
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pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/IL33 protein, human,
http://linkedlifedata.com/resource/pubmed/chemical/Interleukin-1,
http://linkedlifedata.com/resource/pubmed/chemical/Interleukins,
http://linkedlifedata.com/resource/pubmed/chemical/Platelet-Derived Growth Factor,
http://linkedlifedata.com/resource/pubmed/chemical/RNA, Small Interfering,
http://linkedlifedata.com/resource/pubmed/chemical/Receptors, Interleukin,
http://linkedlifedata.com/resource/pubmed/chemical/platelet-derived growth factor BB
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pubmed:status |
MEDLINE
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pubmed:month |
Oct
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pubmed:issn |
1522-1547
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pubmed:author | |
pubmed:issnType |
Electronic
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pubmed:volume |
299
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
G821-32
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pubmed:meshHeading |
pubmed-meshheading:20689058-Animals,
pubmed-meshheading:20689058-Cell Movement,
pubmed-meshheading:20689058-Cell Nucleus,
pubmed-meshheading:20689058-Cell Proliferation,
pubmed-meshheading:20689058-Cells, Cultured,
pubmed-meshheading:20689058-Gene Expression Regulation,
pubmed-meshheading:20689058-Humans,
pubmed-meshheading:20689058-Interleukin-1,
pubmed-meshheading:20689058-Interleukins,
pubmed-meshheading:20689058-Male,
pubmed-meshheading:20689058-Pancreas,
pubmed-meshheading:20689058-Platelet-Derived Growth Factor,
pubmed-meshheading:20689058-RNA, Small Interfering,
pubmed-meshheading:20689058-RNA Interference,
pubmed-meshheading:20689058-Rats,
pubmed-meshheading:20689058-Rats, Wistar,
pubmed-meshheading:20689058-Receptors, Interleukin,
pubmed-meshheading:20689058-Signal Transduction
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pubmed:year |
2010
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pubmed:articleTitle |
Nuclear expression of interleukin-33 in pancreatic stellate cells.
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pubmed:affiliation |
Tohoku Univ. Graduate School of Medicine, Sendai, Japan. amasamune@med.tohoku.ac.jp
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pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
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