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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
5996
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pubmed:dateCreated |
2010-9-3
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pubmed:abstractText |
A family of bacterial effectors including Cif homolog from Burkholderia pseudomallei (CHBP) and Cif from Enteropathogenic Escherichia coli (EPEC) adopt a functionally important papain-like hydrolytic fold. We show here that CHBP was a potent inhibitor of the eukaryotic ubiquitination pathway. CHBP acted as a deamidase that specifically and efficiently deamidated Gln40 in ubiquitin and ubiquitin-like protein NEDD8 both in vitro and during Burkholderia infection. Deamidated ubiquitin was impaired in supporting ubiquitin-chain synthesis. Cif selectively deamidated NEDD8, which abolished the activity of neddylated Cullin-RING ubiquitin ligases (CRLs). Ubiquitination and ubiquitin-dependent degradation of multiple CRL substrates were impaired by Cif in EPEC-infected cells. Mutations of substrate-contacting residues in Cif abolished or attenuated EPEC-induced cytopathic phenotypes of cell cycle arrest and actin stress fiber formation.
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pubmed:grant |
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pubmed:commentsCorrections |
http://linkedlifedata.com/resource/pubmed/commentcorrection/20688984-10802622,
http://linkedlifedata.com/resource/pubmed/commentcorrection/20688984-10921923,
http://linkedlifedata.com/resource/pubmed/commentcorrection/20688984-11030657,
http://linkedlifedata.com/resource/pubmed/commentcorrection/20688984-12062101,
http://linkedlifedata.com/resource/pubmed/commentcorrection/20688984-12705856,
http://linkedlifedata.com/resource/pubmed/commentcorrection/20688984-14651638,
http://linkedlifedata.com/resource/pubmed/commentcorrection/20688984-15737728,
http://linkedlifedata.com/resource/pubmed/commentcorrection/20688984-15989957,
http://linkedlifedata.com/resource/pubmed/commentcorrection/20688984-16753028,
http://linkedlifedata.com/resource/pubmed/commentcorrection/20688984-17136086,
http://linkedlifedata.com/resource/pubmed/commentcorrection/20688984-17157551,
http://linkedlifedata.com/resource/pubmed/commentcorrection/20688984-17257058,
http://linkedlifedata.com/resource/pubmed/commentcorrection/20688984-17310145,
http://linkedlifedata.com/resource/pubmed/commentcorrection/20688984-17535899,
http://linkedlifedata.com/resource/pubmed/commentcorrection/20688984-17719540,
http://linkedlifedata.com/resource/pubmed/commentcorrection/20688984-17943119,
http://linkedlifedata.com/resource/pubmed/commentcorrection/20688984-17952043,
http://linkedlifedata.com/resource/pubmed/commentcorrection/20688984-18005678,
http://linkedlifedata.com/resource/pubmed/commentcorrection/20688984-18284575,
http://linkedlifedata.com/resource/pubmed/commentcorrection/20688984-18687899,
http://linkedlifedata.com/resource/pubmed/commentcorrection/20688984-18705694,
http://linkedlifedata.com/resource/pubmed/commentcorrection/20688984-18779342,
http://linkedlifedata.com/resource/pubmed/commentcorrection/20688984-18802447,
http://linkedlifedata.com/resource/pubmed/commentcorrection/20688984-18845161,
http://linkedlifedata.com/resource/pubmed/commentcorrection/20688984-18851830,
http://linkedlifedata.com/resource/pubmed/commentcorrection/20688984-18997779,
http://linkedlifedata.com/resource/pubmed/commentcorrection/20688984-19194658,
http://linkedlifedata.com/resource/pubmed/commentcorrection/20688984-19225106,
http://linkedlifedata.com/resource/pubmed/commentcorrection/20688984-19262675,
http://linkedlifedata.com/resource/pubmed/commentcorrection/20688984-19308257,
http://linkedlifedata.com/resource/pubmed/commentcorrection/20688984-19325622,
http://linkedlifedata.com/resource/pubmed/commentcorrection/20688984-19369209,
http://linkedlifedata.com/resource/pubmed/commentcorrection/20688984-19782033,
http://linkedlifedata.com/resource/pubmed/commentcorrection/20688984-20064473,
http://linkedlifedata.com/resource/pubmed/commentcorrection/20688984-9192900,
http://linkedlifedata.com/resource/pubmed/commentcorrection/20688984-9192901,
http://linkedlifedata.com/resource/pubmed/commentcorrection/20688984-9438836
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/Amidohydrolases,
http://linkedlifedata.com/resource/pubmed/chemical/Bacterial Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/Cif protein, E coli,
http://linkedlifedata.com/resource/pubmed/chemical/Cullin Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/Escherichia coli Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/Glutamine,
http://linkedlifedata.com/resource/pubmed/chemical/NEDD8 protein, human,
http://linkedlifedata.com/resource/pubmed/chemical/Ubiquitin,
http://linkedlifedata.com/resource/pubmed/chemical/Ubiquitin C,
http://linkedlifedata.com/resource/pubmed/chemical/Ubiquitin-Conjugating Enzymes,
http://linkedlifedata.com/resource/pubmed/chemical/Ubiquitin-Protein Ligases,
http://linkedlifedata.com/resource/pubmed/chemical/Ubiquitins
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pubmed:status |
MEDLINE
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pubmed:month |
Sep
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pubmed:issn |
1095-9203
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pubmed:author |
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pubmed:issnType |
Electronic
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pubmed:day |
3
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pubmed:volume |
329
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
1215-8
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pubmed:dateRevised |
2011-7-25
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pubmed:meshHeading |
pubmed-meshheading:20688984-Humans,
pubmed-meshheading:20688984-Glutamine,
pubmed-meshheading:20688984-Amidohydrolases,
pubmed-meshheading:20688984-Bacterial Proteins,
pubmed-meshheading:20688984-HeLa Cells,
pubmed-meshheading:20688984-Cell Line,
pubmed-meshheading:20688984-Cell Cycle,
pubmed-meshheading:20688984-Escherichia coli Proteins,
pubmed-meshheading:20688984-Burkholderia pseudomallei,
pubmed-meshheading:20688984-Transfection,
pubmed-meshheading:20688984-Stress Fibers,
pubmed-meshheading:20688984-Ubiquitins,
pubmed-meshheading:20688984-Ubiquitin-Protein Ligases
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