Source:http://linkedlifedata.com/resource/pubmed/id/20687159
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Predicate | Object |
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rdf:type | |
lifeskim:mentions | |
pubmed:issue |
1
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pubmed:dateCreated |
2010-12-24
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pubmed:abstractText |
Hypophosphatasia (HPP) is an inherited systemic skeletal disease caused by mutations in the gene encoding the tissue-nonspecific alkaline phosphatase (TNALP) isozyme. The clinical severity of HPP varies widely, with symptoms including rickets and osteomalacia. TNALP knockout (Akp2(-/-)) mice phenotypically mimic the severe infantile form of HPP; that is, TNALP-deficient mice are born with a normal appearance but die by 20 days of age owing to growth failure, hypomineralization, and epileptic seizures. In this study, a lentiviral vector expressing a bone-targeted form of TNALP was injected into the jugular vein of newborn Akp2(-/-) mice. We found that alkaline phosphatase activity in the plasma of treated Akp2(-/-) mice increased and remained at high levels throughout the life of the animals. The treated Akp2(-/-) mice survived for more than 10 months and demonstrated normal physical activity and a healthy appearance. Epileptic seizures were completely inhibited in the treated Akp2(-/-) mice, and X-ray examination of the skeleton showed that mineralization was significantly improved by the gene therapy. These results show that severe infantile HPP in TNALP knockout mice can be treated with a single injection of lentiviral vector during the neonatal period.
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pubmed:grant | |
pubmed:language |
eng
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pubmed:journal | |
pubmed:citationSubset |
IM
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pubmed:chemical | |
pubmed:status |
MEDLINE
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pubmed:month |
Jan
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pubmed:issn |
1523-4681
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pubmed:author | |
pubmed:copyrightInfo |
© 2011 American Society for Bone and Mineral Research.
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pubmed:issnType |
Electronic
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pubmed:volume |
26
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
135-42
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pubmed:dateRevised |
2011-6-17
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pubmed:meshHeading |
pubmed-meshheading:20687159-Alkaline Phosphatase,
pubmed-meshheading:20687159-Animals,
pubmed-meshheading:20687159-Foot,
pubmed-meshheading:20687159-Gene Therapy,
pubmed-meshheading:20687159-Hypophosphatasia,
pubmed-meshheading:20687159-Lentivirus,
pubmed-meshheading:20687159-Mice,
pubmed-meshheading:20687159-Phenotype,
pubmed-meshheading:20687159-Survival Analysis,
pubmed-meshheading:20687159-Tibia
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pubmed:year |
2011
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pubmed:articleTitle |
Prolonged survival and phenotypic correction of Akp2(-/-) hypophosphatasia mice by lentiviral gene therapy.
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pubmed:affiliation |
Department of Biochemistry and Molecular Biology, Nippon Medical School, Tokyo, Japan.
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pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't,
Research Support, N.I.H., Extramural
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