Linked Life Data

20686965

Source:http://linkedlifedata.com/resource/pubmed/id/20686965

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PredicateObject
rdf:type
lifeskim:mentions
pubmed:dateCreated
2010-8-5
pubmed:abstractText
The use of gene constructs for DNA immunization offers several potential advantages over other commonly used vaccine approaches: (1) full-length cDNA provides multiple potential class I and class II epitopes, thus bypassing limitations of MHC restriction; (2) bacterial plasmid DNA contains immunogenic unmethylated CpG motifs (immunostimulatory sequences) that may act as a potent immunological adjuvant; and (3) DNA is relatively simple to purify in large quantities. The cDNA encoding the antigen of interest is cloned into a bacterial expression plasmid with a constitutively active promoter and this plasmid is injected into the skin or muscle where it is taken up by professional antigen-presenting cells, particularly dendritic cells, either through direct transfection or cross-priming. One can further enhance or modulate the immune response through co-delivery of DNA encoding cytokines or chemokines, including cytokine-Fc fusion molecules. The latter use molecular techniques to fuse a cytokine to the Fc portion of IgG1, creating a chimeric molecule with functional activity. In the present chapter, we will outline the approach to develop cytokine-Fc fusion genes as molecular adjuvants and will use GM-CSF as an example.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:issn
1940-6029
pubmed:author
pubmed:issnType
Electronic
pubmed:volume
651
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
131-55
pubmed:meshHeading
pubmed:year
2010
pubmed:articleTitle
Cytokine-FC fusion genes as molecular adjuvants for DNA vaccines.
pubmed:affiliation
Memorial Sloan-Kettering Cancer Center and Weill Medical College of Cornell University, New York, NY, USA.
pubmed:publicationType
Journal Article