Linked Life Data

20686951

Source:http://linkedlifedata.com/resource/pubmed/id/20686951

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:dateCreated
2010-8-5
pubmed:abstractText
Single-cell manipulation supporting robot (SMSR) has enabled femtoinjection, a high-throughput and semi-quantitative microinjection in the range of femtogram (fg) DNA and other molecules. An enhanced green fluorescent protein (EGFP) gene expression vector can be introduced directly into mouse embryonic stem cells at 100 cells/h with a 10% success rate. The intensity of EGFP fluorescence in single ES cells or single colonies of ES cells increases as the concentration of an EGFP gene expression vector in the injection capillary increases from 1 to 50 ng/muL. On the other hand, the knockdown of EGFP gene expression can be demonstrated by femtoinjection of siRNA against EGFP or an shRNA expression vector using an EGFP expressing ES cell line. Femotoinjection can provide a useful method for quantitative analysis of transient gene expression in single cells using RNAi.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:issn
1940-6029
pubmed:author
pubmed:issnType
Electronic
pubmed:volume
650
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
155-70
pubmed:meshHeading
pubmed:year
2010
pubmed:articleTitle
Semi-quantitative analysis of transient single-cell gene expression in embryonic stem cells by femtoinjection.
pubmed:affiliation
Department of Biotechnology and Life Science, Tokyo University of Agriculture and Technology, Tokyo, Japan.
pubmed:publicationType
Journal Article, Research Support, Non-U.S. Gov't