Linked Life Data

20683138

Source:http://linkedlifedata.com/resource/pubmed/id/20683138

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
34
pubmed:dateCreated
2010-8-5
pubmed:abstractText
The effective biofunctionalization of nanoparticles is crucial for biomedical applications. In this study we investigated the covalent biofunctionalization of magnetic nanoparticles based on carbodiimide activation. An important aspect in the covalent biofunctionalization of nanoparticles has been neglected, namely pre-concentration. Exploiting the electrostatic attraction forces between a protein and the nanoparticle surface will favor the covalent immobilization. We showed that low ionic strength buffers with a pH slightly lower than the pI of the selected biomolecules is needed to increase the yield of covalent immobilization. Additionally, it is demonstrated that the covalently immobilized proteins are bioactive, relying on a sandwich assay using gold nanoparticles as reporter labels.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Aug
pubmed:issn
1361-6528
pubmed:author
pubmed:issnType
Electronic
pubmed:day
27
pubmed:volume
21
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
345102
pubmed:meshHeading
pubmed:year
2010
pubmed:articleTitle
Impact of pre-concentration to covalently biofunctionalize suspended nanoparticles.
pubmed:affiliation
Imec, SSET/Functional Nanosystems, Leuven, Belgium. Hilde.Jans@imec.be
pubmed:publicationType
Journal Article, Research Support, Non-U.S. Gov't