Linked Life Data

20682787

Source:http://linkedlifedata.com/resource/pubmed/id/20682787

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
41
pubmed:dateCreated
2010-10-4
pubmed:abstractText
We have crossed ERp57(flx/flx) mice with commercially available mice expressing villin-driven cre-recombinase. Lysates of intestinal epithelial cells were prepared from knock-out (KO) mice and littermates (LM) and used in Western blot analyses with Ab099 against the N terminus of the 1,25D(3)-MARRS (membrane-associated, rapid response steroid-binding) receptor: LM mice exhibited one positive band, which was absent in preparations from KO mice. Saturation analyses of cell lysates with [(3)H]1,25D(3) revealed negligible binding in preparations from either female or male KOs. Lysates from female and male LM mice had similar affinities but different numbers of binding sites. Isolated enterocytes were tested for steroid-stimulated calcium uptake. Treatment of cells from female or male LM mice with 1,25D(3) elicited enhanced calcium uptake in females and males within 5 min. Intestinal cells from KO mice exhibited a severely blunted or completely absent response to hormone. Confocal microscopy of intestinal cells revealed the presence of cell surface vitamin D receptors. However, antibodies to the vitamin D receptor failed to block 1,25D(3)-stimulated calcium uptake. In chick enterocytes we have found that the PKA pathway mediates calcium uptake. The time course for activation of PKA in mouse enterocytes paralleled that for enhanced calcium uptake and for LM females reached 250% of controls within 5 min, and 150% of controls in cells prepared from LM males. Enterocytes from female or male KO mice failed to exhibit steroid hormone-stimulated PKA activity, but did respond to forskolin with enhanced calcium uptake. We conclude that the 1,25D(3)-MARRS receptor is of central importance to steroid hormone-stimulated calcium uptake in mammalian intestinal cells.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Oct
pubmed:issn
1083-351X
pubmed:author
pubmed:issnType
Electronic
pubmed:day
8
pubmed:volume
285
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
31859-66
pubmed:dateRevised
2011-10-10
pubmed:meshHeading
pubmed-meshheading:20682787-Animals, pubmed-meshheading:20682787-Calcitriol, pubmed-meshheading:20682787-Calcium, pubmed-meshheading:20682787-Cells, Cultured, pubmed-meshheading:20682787-Chickens, pubmed-meshheading:20682787-Cyclic AMP-Dependent Protein Kinases, pubmed-meshheading:20682787-Enterocytes, pubmed-meshheading:20682787-Enzyme Activation, pubmed-meshheading:20682787-Female, pubmed-meshheading:20682787-Forskolin, pubmed-meshheading:20682787-Gonadal Steroid Hormones, pubmed-meshheading:20682787-Intestinal Absorption, pubmed-meshheading:20682787-Intestine, Small, pubmed-meshheading:20682787-Male, pubmed-meshheading:20682787-Mice, pubmed-meshheading:20682787-Mice, Inbred BALB C, pubmed-meshheading:20682787-Mice, Knockout, pubmed-meshheading:20682787-Protein Binding, pubmed-meshheading:20682787-Protein Disulfide-Isomerases, pubmed-meshheading:20682787-Vitamins
pubmed:year
2010
pubmed:articleTitle
Intestinal cell calcium uptake and the targeted knockout of the 1,25D3-MARRS (membrane-associated, rapid response steroid-binding) receptor/PDIA3/Erp57.
pubmed:affiliation
Department of Nutrition, Utah State University, Logan, Utah 84322, USA. ilka.nemere@usu.edu
pubmed:publicationType
Journal Article, Research Support, Non-U.S. Gov't