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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
3
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pubmed:dateCreated |
2010-8-4
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pubmed:abstractText |
At 150 kDa, antibodies of the IgG class are too large for their structure to be determined with current NMR methodologies. Because of hinge-region flexibility, it is difficult to obtain atomic-level structural information from the crystal, and questions regarding antibody structure and dynamics in solution remain unaddressed. Here we describe the construction of a model of a human IgG1 monoclonal antibody (trastuzumab) from the crystal structures of fragments. We use a combination of molecular-dynamics (MD) simulation, continuum hydrodynamics modeling, and experimental diffusion measurements to explore antibody behavior in aqueous solution. Hydrodynamic modeling provides a link between the atomic-level details of MD simulation and the size- and shape-dependent data provided by hydrodynamic measurements. Eight independent 40 ns MD trajectories were obtained with the AMBER program suite. The ensemble average of the computed transport properties over all of the MD trajectories agrees remarkably well with the value of the translational diffusion coefficient obtained with dynamic light scattering at 20 degrees C and 27 degrees C, and the intrinsic viscosity measured at 20 degrees C. Therefore, our MD results likely represent a realistic sampling of the conformational space that an antibody explores in aqueous solution.
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pubmed:grant |
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pubmed:commentsCorrections |
http://linkedlifedata.com/resource/pubmed/commentcorrection/20682269-1004567,
http://linkedlifedata.com/resource/pubmed/commentcorrection/20682269-10064707,
http://linkedlifedata.com/resource/pubmed/commentcorrection/20682269-10211534,
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http://linkedlifedata.com/resource/pubmed/commentcorrection/20682269-15264254,
http://linkedlifedata.com/resource/pubmed/commentcorrection/20682269-15267980,
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http://linkedlifedata.com/resource/pubmed/commentcorrection/20682269-19527731,
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http://linkedlifedata.com/resource/pubmed/commentcorrection/20682269-9892632
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
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pubmed:status |
MEDLINE
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pubmed:month |
Aug
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pubmed:issn |
1542-0086
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pubmed:author |
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pubmed:copyrightInfo |
2010 Biophysical Society. Published by Elsevier Inc. All rights reserved.
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pubmed:issnType |
Electronic
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pubmed:day |
4
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pubmed:volume |
99
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
905-13
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pubmed:dateRevised |
2011-11-17
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pubmed:meshHeading |
pubmed-meshheading:20682269-Antibodies, Monoclonal,
pubmed-meshheading:20682269-Antibodies, Monoclonal, Humanized,
pubmed-meshheading:20682269-Diffusion,
pubmed-meshheading:20682269-Immunoglobulin G,
pubmed-meshheading:20682269-Light,
pubmed-meshheading:20682269-Molecular Dynamics Simulation,
pubmed-meshheading:20682269-Reproducibility of Results,
pubmed-meshheading:20682269-Rotation,
pubmed-meshheading:20682269-Scattering, Radiation,
pubmed-meshheading:20682269-Water
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pubmed:year |
2010
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pubmed:articleTitle |
Construction, MD simulation, and hydrodynamic validation of an all-atom model of a monoclonal IgG antibody.
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pubmed:affiliation |
Department of Early Stage Pharmaceutical Development, Genentech, South San Francisco, California, USA.
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pubmed:publicationType |
Journal Article,
Research Support, N.I.H., Extramural
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