Linked Life Data

20681513

Source:http://linkedlifedata.com/resource/pubmed/id/20681513

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
17
pubmed:dateCreated
2010-8-31
pubmed:abstractText
A unique capability of redox-magnetohydrodynamics (redox-MHD) for handling liquids on a small scale was demonstrated. A 1.2 muL solution plug was pumped from an injection site to a detector without the need for a channel to direct the flow. The redox pumping species did not interfere with enzymatic activity in a solution compatible with enzyme-linked immunoassays. Alkaline phosphatase (AP), a common enzyme label, converted p-aminophenyl phosphate (PAPP) to p-aminophenol (PAP(R)) in the presence of 2.5 mM Ru(NH(3))(6)Cl(2) and 2.5 mM Ru(NH(3))(6) Cl(3), in 0.1 M Tris buffer (pH = 9). A solution plug containing PAPP (no AP) was pumped through the surrounding solution containing AP (no PAPP), and the enzymatically generated PAP(R) was easily detected and distinguishable electrochemically from the pumping species with square wave voltammetry down to 0.1 mM concentrations. The test device consisted of a silicon chip containing individually addressable microband electrodes, placed on a 0.5 T NdFeB permanent magnet with the field oriented perpendicular to the chip. A 8.0 mm wide x 15.5 mm long x 1.5 mm high volume of solution was contained by a poly(dimethylsiloxane) gasket and capped with a glass slide. A steady-state fluid velocity of approximately 30 mum/s was generated in a reinforcing flow configuration between oppositely polarized sets of pumping electrodes with approximately 2.1 muA.
pubmed:grant
pubmed:commentsCorrections
http://linkedlifedata.com/resource/pubmed/commentcorrection/20681513-11192117, http://linkedlifedata.com/resource/pubmed/commentcorrection/20681513-12139035, http://linkedlifedata.com/resource/pubmed/commentcorrection/20681513-15080758, http://linkedlifedata.com/resource/pubmed/commentcorrection/20681513-15100815, http://linkedlifedata.com/resource/pubmed/commentcorrection/20681513-15100857, http://linkedlifedata.com/resource/pubmed/commentcorrection/20681513-15854821, http://linkedlifedata.com/resource/pubmed/commentcorrection/20681513-16737232, http://linkedlifedata.com/resource/pubmed/commentcorrection/20681513-17124540, http://linkedlifedata.com/resource/pubmed/commentcorrection/20681513-17129069, http://linkedlifedata.com/resource/pubmed/commentcorrection/20681513-17487976, http://linkedlifedata.com/resource/pubmed/commentcorrection/20681513-20210341, http://linkedlifedata.com/resource/pubmed/commentcorrection/20681513-20380431, http://linkedlifedata.com/resource/pubmed/commentcorrection/20681513-6164387, http://linkedlifedata.com/resource/pubmed/commentcorrection/20681513-8017631, http://linkedlifedata.com/resource/pubmed/commentcorrection/20681513-8328672
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Sep
pubmed:issn
1520-6882
pubmed:author
pubmed:issnType
Electronic
pubmed:day
1
pubmed:volume
82
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
7068-72
pubmed:dateRevised
2011-9-13
pubmed:meshHeading
pubmed:year
2010
pubmed:articleTitle
Redox-magnetohydrodynamic microfluidics without channels and compatible with electrochemical detection under immunoassay conditions.
pubmed:affiliation
Department of Chemistry and Biochemistry, University of Arkansas, Fayetteville, Arkansas 72701, USA.
pubmed:publicationType
Journal Article, Research Support, U.S. Gov't, Non-P.H.S., Research Support, N.I.H., Extramural