Source:http://linkedlifedata.com/resource/pubmed/id/20678559
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| Predicate | Object |
|---|---|
| rdf:type | |
| lifeskim:mentions | |
| pubmed:issue |
3
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| pubmed:dateCreated |
2010-9-14
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| pubmed:abstractText |
o,p'-Dichlorodiphenyltrichloroethane (o,p'-DDT) is a DDT isomer and xenoestrogen that can induce inflammation and cancer. However, the effect of o,p'-DDT on aromatase is unclear. Thus, we investigated the effects of o,p'-DDT on aromatase expression in human breast cancer cells. We also examined whether cyclooxygenase-2 (COX-2) is involved in o,p'-DDT-mediated aromatase expression. Treatment with o,p'-DDT-induced aromatase protein expression in MCF-7 and MDA-MB-231 human breast cancer cells; enhancing aromatase gene expression, and enzyme and promoter activity. Treatment with ICI 182.780, a estrogen receptor antagonist, did not affect the inductive effects of o,p'-DDT on aromatase expression. In addition, o,p'-DDT increased COX-2 protein levels markedly, increased COX-2 mRNA expression and promoter activity, enhanced the production of prostaglandin E(2) (PGE(2)), induced cyclic AMP response element (CRE) activation, and cAMP levels and binding of CREB. o,p'-DDT also increased the phosphorylation of PKA, Akt, ERK, and JNK in their signaling pathways in MCF-7 and MDA-MB-231 cells. Finally, o,p'-DDT induction of aromatase was inhibited by various inhibitors [COX-2 (by NS-398), PKA (H-89), PI3-K/Akt (LY 294002), EP2 (AH6809), and EP4 receptor (AH23848)]. Together, these results suggest that o,p'-DDT increases aromatase, and that o,p'-DDT-induced aromatase is correlated with COX-2 up-regulation, mediated via the CRE activation and PKA and PI3-kinase/Akt signaling pathways in breast cancer cells.
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| pubmed:language |
eng
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| pubmed:journal | |
| pubmed:citationSubset |
IM
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| pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/Aromatase,
http://linkedlifedata.com/resource/pubmed/chemical/CREB-Binding Protein,
http://linkedlifedata.com/resource/pubmed/chemical/CREBBP protein, human,
http://linkedlifedata.com/resource/pubmed/chemical/Cyclooxygenase 2,
http://linkedlifedata.com/resource/pubmed/chemical/DDT,
http://linkedlifedata.com/resource/pubmed/chemical/Dinoprostone,
http://linkedlifedata.com/resource/pubmed/chemical/Enzyme Inhibitors,
http://linkedlifedata.com/resource/pubmed/chemical/PTGER4 protein, human,
http://linkedlifedata.com/resource/pubmed/chemical/RNA,
http://linkedlifedata.com/resource/pubmed/chemical/Receptors, Estrogen,
http://linkedlifedata.com/resource/pubmed/chemical/Receptors, Prostaglandin E,
http://linkedlifedata.com/resource/pubmed/chemical/Receptors, Prostaglandin E, EP4...,
http://linkedlifedata.com/resource/pubmed/chemical/o,p'-DDT
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| pubmed:status |
MEDLINE
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| pubmed:month |
Oct
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| pubmed:issn |
1879-3169
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| pubmed:author | |
| pubmed:copyrightInfo |
Copyright 2010 Elsevier Ireland Ltd. All rights reserved.
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| pubmed:issnType |
Electronic
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| pubmed:day |
20
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| pubmed:volume |
198
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| pubmed:owner |
NLM
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| pubmed:authorsComplete |
Y
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| pubmed:pagination |
331-41
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| pubmed:dateRevised |
2010-11-18
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| pubmed:meshHeading |
pubmed-meshheading:20678559-Aromatase,
pubmed-meshheading:20678559-Blotting, Western,
pubmed-meshheading:20678559-Breast Neoplasms,
pubmed-meshheading:20678559-CREB-Binding Protein,
pubmed-meshheading:20678559-Cell Line, Tumor,
pubmed-meshheading:20678559-Cyclooxygenase 2,
pubmed-meshheading:20678559-DDT,
pubmed-meshheading:20678559-Dinoprostone,
pubmed-meshheading:20678559-Enzyme Activation,
pubmed-meshheading:20678559-Enzyme Inhibitors,
pubmed-meshheading:20678559-Female,
pubmed-meshheading:20678559-Humans,
pubmed-meshheading:20678559-RNA,
pubmed-meshheading:20678559-Receptors, Estrogen,
pubmed-meshheading:20678559-Receptors, Prostaglandin E,
pubmed-meshheading:20678559-Receptors, Prostaglandin E, EP4 Subtype,
pubmed-meshheading:20678559-Reverse Transcriptase Polymerase Chain Reaction,
pubmed-meshheading:20678559-Signal Transduction
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| pubmed:year |
2010
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| pubmed:articleTitle |
The role of cyclooxygenase-2-dependent signaling via cyclic AMP response element activation on aromatase up-regulation by o,p'-DDT in human breast cancer cells.
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| pubmed:affiliation |
Department of Toxicology, College of Pharmacy, Chungnam National University, 220 Gung-dong, Daejeon 305-764, Yuseong-Gu, South Korea.
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| pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
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