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PredicateObject
rdf:type
lifeskim:mentions
pubmed:dateCreated
2010-8-2
pubmed:abstractText
The expansion of the genetic alphabet, by an unnatural base pair system, provides a powerful tool for the site-specific incorporation of extra, functional components into nucleic acids by replication and transcription. We developed several unnatural base pairs that function in PCR and in vitro transcription. Among them, a hydrophobic, unnatural base pair between 7-(2-thienyl)-imidazo[4,5-b]pyridine (denoted by Ds) and pyrrole-2-carbaldehyde (denoted by Pa) exhibits high fidelity in PCR and T7 transcription. Modified Pa bases linked with functional groups of interest can also be site-specifically incorporated into RNA opposite Ds in DNA templates, by T7 RNA polymerase. Here, we describe the methods for the site-specific biotinylation of RNA molecules by transcription using the Ds-Pa pair with biotinylated PaTP (Biotin-PaTP).
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:issn
1940-6029
pubmed:author
pubmed:issnType
Electronic
pubmed:volume
634
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
355-69
pubmed:meshHeading
pubmed:year
2010
pubmed:articleTitle
Site-specific incorporation of extra components into RNA by transcription using unnatural base pair systems.
pubmed:affiliation
Systems and Structural Biology Center, RIKEN, and TagCyx Biotechnologies, Yokohama, Kanagawa, Japan.
pubmed:publicationType
Journal Article, Research Support, Non-U.S. Gov't