Source:http://linkedlifedata.com/resource/pubmed/id/20676996
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rdf:type | |
lifeskim:mentions | |
pubmed:dateCreated |
2010-8-2
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pubmed:abstractText |
The expansion of the genetic alphabet, by an unnatural base pair system, provides a powerful tool for the site-specific incorporation of extra, functional components into nucleic acids by replication and transcription. We developed several unnatural base pairs that function in PCR and in vitro transcription. Among them, a hydrophobic, unnatural base pair between 7-(2-thienyl)-imidazo[4,5-b]pyridine (denoted by Ds) and pyrrole-2-carbaldehyde (denoted by Pa) exhibits high fidelity in PCR and T7 transcription. Modified Pa bases linked with functional groups of interest can also be site-specifically incorporated into RNA opposite Ds in DNA templates, by T7 RNA polymerase. Here, we describe the methods for the site-specific biotinylation of RNA molecules by transcription using the Ds-Pa pair with biotinylated PaTP (Biotin-PaTP).
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pubmed:language |
eng
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pubmed:journal | |
pubmed:citationSubset |
IM
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pubmed:chemical | |
pubmed:status |
MEDLINE
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pubmed:issn |
1940-6029
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pubmed:author | |
pubmed:issnType |
Electronic
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pubmed:volume |
634
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
355-69
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pubmed:meshHeading | |
pubmed:year |
2010
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pubmed:articleTitle |
Site-specific incorporation of extra components into RNA by transcription using unnatural base pair systems.
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pubmed:affiliation |
Systems and Structural Biology Center, RIKEN, and TagCyx Biotechnologies, Yokohama, Kanagawa, Japan.
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pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
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