Linked Life Data

20675897

Source:http://linkedlifedata.com/resource/pubmed/id/20675897

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
2-4
pubmed:dateCreated
2010-8-2
pubmed:abstractText
Previous reports have demonstrated that red blood cells (RBC) have an active nitric oxide (NO) synthesizing mechanism which has properties similar to endothelial nitric oxide synthase (eNOS). This red cell NOS activity contributes to the NO export from RBC. The present study explored the influence of shear stress applied to RBC on NO concentrations of cell suspensions. RBC were exposed to shear stress by filtration through 5 microm diameter pores under 10 cm H2O pressure, generating a wall shear stress of approximately 110Pa. NO concentration in the RBC suspensions were measured using electrochemical NO probes before and after filtration through the micropores. NO concentration was found to be significantly increased after a single passage of RBC suspensions through the micropores. The increment in NO concentration depended on the presence of calcium, being 21.8+/-4.4 nM with 1 mM calcium and 13.7+/-2.7 nM without added calcium. Including the calcium chelator EDTA completely abolished this increase. The increment of NO was also affected by the level of oxygenation, being more pronounced under hypoxic conditions. These results confirm that RBC NO generating mechanisms can be stimulated by exposing red cells to shear stress and that calcium plays a role in this stimulation.
pubmed:grant
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:issn
1875-8622
pubmed:author
pubmed:issnType
Electronic
pubmed:volume
45
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
169-75
pubmed:meshHeading
pubmed:year
2010
pubmed:articleTitle
Nitric oxide generation in red blood cells induced by mechanical stress.
pubmed:affiliation
Department of Physiology, Akdeniz University, Antalya, Turkey.
pubmed:publicationType
Journal Article, Research Support, Non-U.S. Gov't, Research Support, N.I.H., Extramural